Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke

被引:48
|
作者
Deng, Qi-Wen [1 ,2 ]
Li, Shuo [1 ]
Wang, Huan [1 ]
Sun, Hui-Ling [3 ]
Zuo, Lei [1 ]
Gu, Zheng-Tian [1 ]
Lu, Guo [4 ]
Sun, Cai-Zhi [5 ]
Zhang, Han-Qing [1 ]
Yan, Fu-Ling [1 ]
机构
[1] Southeast Univ, Sch Med, Affiliated ZhongDa Hosp, Dept Neurol, Nanjing 210009, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Hosp 1, Dept Neurol, Nanjing 210006, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Nanjing Hosp 1, Gen Clin Res Ctr, Nanjing 210006, Jiangsu, Peoples R China
[4] Dezhou Peoples Hosp, Dept Neurol, Dezhou 253014, Peoples R China
[5] Nanjing Med Univ, Nanjing Hosp 1, Dept Emergency, Nanjing 210006, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
HEALTH-CARE PROFESSIONALS; GENE-EXPRESSION; DIAGNOSIS; BIOMARKER; ASSOCIATION; DEFINITION; STATEMENT; OUTCOMES; LNCRNAS; ATTACK;
D O I
10.1042/CS20180411
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of the present study was to investigate the expression profile of lncRNAs in peripheral blood mononuclear cells (PBMCs) of acute IS patients and to explore their utility as biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination index) was established using logistic regression. LncRNA microarray analysis showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 remained increased, indicating the ability of lncRNAs to monitor IS dynamics. Receiver-operating characteristic (ROC) curve analysis showed that the lncRNA-based combination index outperformed serum brain-derived neurotrophic factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients from TIA patients and HCs with areas under ROC curve of more than 0.84. Furthermore, the combination index increased significantly after treatment and was correlated with neurological deficit severity of IS. The panel of these altered lncRNAs was associated with acute IS and could serve as a novel diagnostic method.
引用
收藏
页码:1597 / 1614
页数:18
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