Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke

被引:48
|
作者
Deng, Qi-Wen [1 ,2 ]
Li, Shuo [1 ]
Wang, Huan [1 ]
Sun, Hui-Ling [3 ]
Zuo, Lei [1 ]
Gu, Zheng-Tian [1 ]
Lu, Guo [4 ]
Sun, Cai-Zhi [5 ]
Zhang, Han-Qing [1 ]
Yan, Fu-Ling [1 ]
机构
[1] Southeast Univ, Sch Med, Affiliated ZhongDa Hosp, Dept Neurol, Nanjing 210009, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Hosp 1, Dept Neurol, Nanjing 210006, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Nanjing Hosp 1, Gen Clin Res Ctr, Nanjing 210006, Jiangsu, Peoples R China
[4] Dezhou Peoples Hosp, Dept Neurol, Dezhou 253014, Peoples R China
[5] Nanjing Med Univ, Nanjing Hosp 1, Dept Emergency, Nanjing 210006, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
HEALTH-CARE PROFESSIONALS; GENE-EXPRESSION; DIAGNOSIS; BIOMARKER; ASSOCIATION; DEFINITION; STATEMENT; OUTCOMES; LNCRNAS; ATTACK;
D O I
10.1042/CS20180411
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of the present study was to investigate the expression profile of lncRNAs in peripheral blood mononuclear cells (PBMCs) of acute IS patients and to explore their utility as biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination index) was established using logistic regression. LncRNA microarray analysis showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 remained increased, indicating the ability of lncRNAs to monitor IS dynamics. Receiver-operating characteristic (ROC) curve analysis showed that the lncRNA-based combination index outperformed serum brain-derived neurotrophic factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients from TIA patients and HCs with areas under ROC curve of more than 0.84. Furthermore, the combination index increased significantly after treatment and was correlated with neurological deficit severity of IS. The panel of these altered lncRNAs was associated with acute IS and could serve as a novel diagnostic method.
引用
收藏
页码:1597 / 1614
页数:18
相关论文
共 50 条
  • [31] PARK7 as a peripheral blood biomarker in ischemic stroke: pathway analysis of its molecular function within peripheral blood mononuclear cells
    Vavougios, G.
    Ioakeimidis, M.
    Doskas, T.
    EUROPEAN JOURNAL OF NEUROLOGY, 2020, 27 : 787 - 788
  • [32] MicroRNAs differential gene expression in peripheral blood mononuclear cells from patients with acute myocarditis
    Marketou, M.
    Kontaraki, J.
    Konstantinou, J.
    Parthenakis, F.
    Nakou, H.
    Touloupaki, M.
    Kochiadakis, G.
    Logakis, J.
    Vougia, D.
    Vardas, P.
    EUROPEAN HEART JOURNAL, 2016, 37 : 1153 - 1153
  • [33] Alpha-synuclein alterations in red blood cells of peripheral blood after acute ischemic stroke
    Wu, Zimu
    Li, Xuying
    Zeng, Minyan
    Qiu, Hongyan
    Feng, Haixia
    Xu, Xiaonan
    Yu, Shun
    Wu, Jun
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2019, 12 (05): : 1757 - 1763
  • [34] Differential Transcriptome of Peripheral Mononuclear Blood Cells in Pulmonary Sarcoidosis
    Yoshioka, K.
    Kawasaki, T.
    Sato, H.
    Ishii, D.
    Imamoto, T.
    Abe, M.
    Hasegawa, Y.
    Ohara, O.
    Suzuki, T.
    Tatsumi, K.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2022, 205
  • [35] Immune activation in the peripheral blood of patients with acute ischemic stroke
    Yan, Jun
    Greer, Judith M.
    Etherington, Kellie
    Cadigan, Greg P.
    Cavanagh, Helen
    Henderson, Robert D.
    O'Sullivan, John D.
    Pandian, Jeyaraj D.
    Read, Stephen J.
    McCombe, Pamela A.
    JOURNAL OF NEUROIMMUNOLOGY, 2009, 206 (1-2) : 112 - 117
  • [36] The identification of sex-specific biomarkers in peripheral blood mononuclear cells from elderly individuals with ischemic stroke
    Qin, L. -H.
    Chen, L.
    Cao, X.
    Huang, T. -J.
    Li, Z. -Y.
    Li, S.
    Wang, G. -Z.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2023, 27 (14) : 6496 - +
  • [37] Long Noncoding RNA Malat1 Regulates Cerebrovascular Pathologies in Ischemic Stroke
    Zhang, Xuejing
    Tang, Xuelian
    Liu, Kai
    Hamblin, Milton H.
    Yin, Ke-Jie
    JOURNAL OF NEUROSCIENCE, 2017, 37 (07): : 1797 - 1806
  • [38] Altered expression of long noncoding RNAs in peripheral blood mononuclear cells in patients with impaired leptomeningeal collaterals after acute anterior large vessel occlusions
    Wu, Qisi
    Le, Ting
    Zhu, Dan
    Lv, Fajin
    Qin, Xinyue
    ANNALS OF TRANSLATIONAL MEDICINE, 2019, 7 (20)
  • [39] Predictive value of long noncoding RNA ZFAS1 in patients with ischemic stroke
    Wang, June
    Ruan, Jie
    Zhu, Man
    Yang, Jiayi
    Du, Shenlin
    Xu, Peipei
    Zhang, Zheng
    Wang, Ping
    Yang, Weiqing
    Yu, Mingxia
    CLINICAL AND EXPERIMENTAL HYPERTENSION, 2019, 41 (07) : 615 - 621
  • [40] Differential Regulation of the Immune System in Peripheral Blood Following Ischemic Stroke
    Liu, Wenhao
    Yang, Xin-Zhuang
    Zhang, Dingding
    He, Xin
    Yu, Qianlan
    Liu, Xinquan
    Dai, Yi
    BIOMED RESEARCH INTERNATIONAL, 2022, 2022