Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke

被引:48
|
作者
Deng, Qi-Wen [1 ,2 ]
Li, Shuo [1 ]
Wang, Huan [1 ]
Sun, Hui-Ling [3 ]
Zuo, Lei [1 ]
Gu, Zheng-Tian [1 ]
Lu, Guo [4 ]
Sun, Cai-Zhi [5 ]
Zhang, Han-Qing [1 ]
Yan, Fu-Ling [1 ]
机构
[1] Southeast Univ, Sch Med, Affiliated ZhongDa Hosp, Dept Neurol, Nanjing 210009, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Hosp 1, Dept Neurol, Nanjing 210006, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Nanjing Hosp 1, Gen Clin Res Ctr, Nanjing 210006, Jiangsu, Peoples R China
[4] Dezhou Peoples Hosp, Dept Neurol, Dezhou 253014, Peoples R China
[5] Nanjing Med Univ, Nanjing Hosp 1, Dept Emergency, Nanjing 210006, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
HEALTH-CARE PROFESSIONALS; GENE-EXPRESSION; DIAGNOSIS; BIOMARKER; ASSOCIATION; DEFINITION; STATEMENT; OUTCOMES; LNCRNAS; ATTACK;
D O I
10.1042/CS20180411
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of the present study was to investigate the expression profile of lncRNAs in peripheral blood mononuclear cells (PBMCs) of acute IS patients and to explore their utility as biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination index) was established using logistic regression. LncRNA microarray analysis showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 remained increased, indicating the ability of lncRNAs to monitor IS dynamics. Receiver-operating characteristic (ROC) curve analysis showed that the lncRNA-based combination index outperformed serum brain-derived neurotrophic factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients from TIA patients and HCs with areas under ROC curve of more than 0.84. Furthermore, the combination index increased significantly after treatment and was correlated with neurological deficit severity of IS. The panel of these altered lncRNAs was associated with acute IS and could serve as a novel diagnostic method.
引用
收藏
页码:1597 / 1614
页数:18
相关论文
共 50 条
  • [1] Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke
    Grond-Ginsbach, C.
    Hummel, M.
    Wiest, T.
    Horstmann, S.
    Pfleger, K.
    Hergenhahn, M.
    Hollstein, M.
    Mansmann, U.
    Grau, A. J.
    Wagner, S.
    JOURNAL OF NEUROLOGY, 2008, 255 (05) : 723 - 731
  • [2] Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke
    C. Grond-Ginsbach
    M. Hummel
    T. Wiest
    S. Horstmann
    K. Pfleger
    M. Hergenhahn†
    M. Hollstein
    U. Mansmann
    A. J. Grau
    S. Wagner
    Journal of Neurology, 2008, 255 : 723 - 731
  • [3] Long Noncoding RNA ENST00000581794.1 in Peripheral Blood Mononuclear Cells Acts as a Novel Biomarker in Patients with Acute Myocardial Infarction
    Xue-He, Z.
    Zhao, Gian
    Song, Ning
    Luo, Jun-Yi
    Qing-Jie, C.
    Chenc, A.
    Tuxun, Munire
    Aihemaiti, G.
    Li, Xiao-Mei
    INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2020, 82 : 48 - 55
  • [4] Genome-wide analysis of long noncoding RNA expression in peripheral blood mononuclear cells of uremia patients
    Sui, Weiguo
    Yan, Qiang
    Li, Huan
    Liu, Juan
    Chen, Jiejing
    Li, Liping
    Dai, Yong
    JOURNAL OF NEPHROLOGY, 2013, 26 (04) : 731 - 738
  • [5] Integrated analysis of mRNA and long noncoding RNA profiles in peripheral blood mononuclear cells of patients with bronchial asthma
    Han Cui
    Ruirui Duan
    Hongtao Niu
    Tao Yu
    Ke Huang
    Chen Chen
    Ke Hao
    Ting Yang
    Chen Wang
    BMC Pulmonary Medicine, 22
  • [6] Integrated analysis of mRNA and long noncoding RNA profiles in peripheral blood mononuclear cells of patients with bronchial asthma
    Cui, Han
    Duan, Ruirui
    Niu, Hongtao
    Yu, Tao
    Huang, Ke
    Chen, Chen
    Hao, Ke
    Yang, Ting
    Wang, Chen
    BMC PULMONARY MEDICINE, 2022, 22 (01)
  • [7] Differential miRNA expressions in peripheral blood mononuclear cells for diagnosis of lung cancer
    Ma, Jie
    Lin, Yanli
    Zhan, Min
    Mann, Dean L.
    Stass, Sanford A.
    Jiang, Feng
    LABORATORY INVESTIGATION, 2015, 95 (10) : 1197 - 1206
  • [8] CircRNA expression profiles and function prediction in peripheral blood mononuclear cells of patients with acute ischemic stroke
    Dong, Zhaofei
    Deng, Lingna
    Peng, Qingxia
    Pan, Jingrui
    Wang, Yidong
    JOURNAL OF CELLULAR PHYSIOLOGY, 2020, 235 (03) : 2609 - 2618
  • [9] Expression profiles of long noncoding RNAs and mRNAs in peripheral blood mononuclear cells of patients with acute myocardial infarction
    Zhao, Pingsen
    Wu, Heming
    Zhong, Zhixiong
    Zhang, Qifeng
    Zhong, Wei
    Li, Bin
    Li, Cunren
    Liu, Zhidong
    Yang, Min
    MEDICINE, 2018, 97 (41)
  • [10] Long noncoding RNA expression in peripheral blood mononuclear cells and suicide risk in Chinese patients with major depressive disorder
    Cui, Xuelian
    Niu, Wei
    Kong, Lingming
    He, Mingjun
    Jiang, Kunhong
    Chen, Shengdong
    Zhong, Aifang
    Li, Wanshuai
    Lu, Jim
    Zhang, Liyi
    BRAIN AND BEHAVIOR, 2017, 7 (06):