Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging

被引：16

作者：

Arai, Yoshiyuki ^{[1
,2
]}

Takauchi, Hiroki ^{[2
]}

Ogami, Yuhei ^{[2
]}

Fujiwara, Satsuki ^{[1
]}

Nakano, Masahiro ^{[1
,2
]}

Matsuda, Tomoki ^{[1
,2
]}

Nagai, Takeharu ^{[1
,2
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, 8-1 Mihogaoka, Ibaraki, Osaka 5670047, Japan

[2] Osaka Univ, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

来源：

ACS CHEMICAL BIOLOGY | 2018年 / 13卷 / 08期

关键词：

DIFFRACTION RESOLUTION LIMIT; MICROSCOPY; BREAKING; BARRIER; STORM;

D O I：

10.1021/acschembio.8b00200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.

引用

页码：1938 / 1943

页数：6

共 50 条

[21] Super-Resolution Live Cell Imaging of Subcellular Structures
Ranjan, Rajesh
Chen, Xin
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021, (167): : 1 - 13
[22] Super-resolution microscopy in live-cell imaging
Hedde, Per Niklas
Gayda, Susan
Nienhaus, Karin
Nienhaus, G. Ulrich
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2011, 40 : 151 - 152
[23] Super-resolution Microscopy Approaches for Live Cell Imaging
Godin, Antoine G.
Lounis, Brahim
Cognet, Laurent
BIOPHYSICAL JOURNAL, 2014, 107 (08) : 1777 - 1784
[24] Rhodamine-Derived Fluorescent Dye with Inherent Blinking Behavior for Super-Resolution Imaging
Macdonald, Patrick J.
Gayda, Susan
Haack, Richard A.
Ruan, Qiaoqiao
Himmelsbach, Richard J.
Tetin, Sergey Y.
ANALYTICAL CHEMISTRY, 2018, 90 (15) : 9165 - 9173
[25] Blinking Carbon Dots as a Super-resolution Imaging Probe
Dolai, Jayanta
Joshi, Prakash
Mondal, Partha Pratim
Maity, Anupam
Mukherjee, Buddhadev
Jana, Nikhil R.
ACS APPLIED MATERIALS & INTERFACES, 2024, 16 (13) : 16003 - 16010
[26] Intrinsic blinking of red fluorescent proteins for super-resolution microscopy
Klementieva, Natalia V.
Pavlikov, Anton I.
Moiseev, Alexander A.
Bozhanova, Nina G.
Mishina, Natalie M.
Lukyanov, Sergey A.
Zagaynova, Elena V.
Lukyanov, Konstantin A.
Mishin, Alexander S.
CHEMICAL COMMUNICATIONS, 2017, 53 (05) : 949 - 951
[27] Blinking Carbon Dots as a Super-resolution Imaging Probe
Dolai, Jayanta
Joshi, Prakash
Mondal, Partha Pratim
Maity, Anupam
Mukherjee, Buddhadev
Jana, Nikhil R.
ACS Applied Materials and Interfaces, 2024, 16 (13): : 16003 - 16010
[28] Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes
Hennig, Simon
van de Linde, Sebastian
Lummer, Martina
Simonis, Matthias
Huser, Thomas
Sauer, Markus
NANO LETTERS, 2015, 15 (02) : 1374 - 1381
[29] Super-resolution imaging in live cells
Cox, Susan
DEVELOPMENTAL BIOLOGY, 2015, 401 (01) : 175 - 181
[30] Super-resolution imaging in live animals
Nature Methods, 2014, 11 (11) : 1091 - 1091

← 1 2 3 4 5 →