Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

被引：18

作者：

Klementieva, Natalia V. ^{[1
]}

Pavlikov, Anton I. ^{[1
]}

Moiseev, Alexander A. ^{[2
]}

Bozhanova, Nina G. ^{[3
]}

Mishina, Natalie M. ^{[1
,3
]}

Lukyanov, Sergey A. ^{[1
,3
,4
]}

Zagaynova, Elena V. ^{[1
]}

Lukyanov, Konstantin A. ^{[1
,3
]}

Mishin, Alexander S. ^{[1
,3
]}

机构：

[1] Nizhny Novgorod State Med Acad, Nizhnii Novgorod, Russia

[2] Inst Appl Phys, Nizhnii Novgorod, Russia

[3] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[4] Pirogov Russian Natl Res Med Univ, Moscow, Russia

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 05期

基金：

俄罗斯科学基金会;

关键词：

FLUCTUATION IMAGING SOFI; LOCALIZATION MICROSCOPY; CORRELATION SPECTROSCOPY; MOLECULES;

D O I：

10.1039/c6cc09200d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-molecule localizationmicroscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

引用

页码：949 / 951

页数：3

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