Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0

被引：26

作者：

Rezzonico, F

Moënne-Loccoz, Y

Défago, G

机构：

[1] Swiss Fed Inst Technol, Swiss Fed Inst Technol, Inst Plant Sci, Phytopathol Grp, CH-8092 Zurich, Switzerland

[2] Univ Lyon 1, UMR CNRS Ecol Micobienne, F-69622 Villeurbanne, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 01期

关键词：

D O I：

10.1128/AEM.69.1.686-690.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.

引用

页码：686 / 690

页数：5