Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0

被引：26
|
作者：
Rezzonico, F
Moënne-Loccoz, Y
Défago, G
机构：
[1] Swiss Fed Inst Technol, Swiss Fed Inst Technol, Inst Plant Sci, Phytopathol Grp, CH-8092 Zurich, Switzerland
[2] Univ Lyon 1, UMR CNRS Ecol Micobienne, F-69622 Villeurbanne, France
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 01期
关键词：
D O I：
10.1128/AEM.69.1.686-690.2003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.
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页码：686 / 690
页数：5
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