The domestication of Cas9

被引：10
|
作者：
Urnov, Fyodor [1 ]
机构：
[1] Sangamo BioSci, Richmond, CA 94804 USA
来源：
NATURE | 2016年 / 529卷 / 7587期
关键词：
GENOME; NUCLEASES;
D O I：
10.1038/529468a
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The enzyme Cas9 is used in genome editing to cut selected DNA sequences, but it also creates breaks at off-target sites. Protein engineering has now been used to make Cas9 enzymes that have minimal off-target effects. See Article p.490
引用
收藏
页码：468 / 469
页数：2
相关论文
共 50 条
  • [31] CAS9 AND THE IMPORTANCE OF ASYMMETRY
    Rusk, Nicole
    NATURE METHODS, 2016, 13 (04) : 286 - 287
  • [32] Cas9 and the importance of asymmetry
    Nicole Rusk
    Nature Methods, 2016, 13 : 287 - 287
  • [33] Responding to CRISPER/Cas9
    Finneran, Kevin
    ISSUES IN SCIENCE AND TECHNOLOGY, 2016, 32 (03) : 33 - 34
  • [34] Expanding the reach of Cas9
    Wrighton, Katharine H.
    NATURE REVIEWS GENETICS, 2018, 19 (05) : 250 - 250
  • [35] RNA targeting by Cas9
    Eytan Zlotorynski
    Nature Reviews Molecular Cell Biology, 2014, 15 (11) : 701 - 701
  • [36] Advances in CRISPR/Cas9
    Zhu, Youmin
    BIOMED RESEARCH INTERNATIONAL, 2022, 2022
  • [37] In Vitro Enzymology of Cas9
    Anders, Carolin
    Jinek, Martin
    USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS, 2014, 546 : 1 - 20
  • [38] Cleaving RNA with Cas9
    Odelia Ghodsizadeh
    Nature Methods, 2014, 11 : 1090 - 1090
  • [39] Smash and DASH with Cas9
    Ramani, Vijay
    Shendure, Jay
    GENOME BIOLOGY, 2016, 17
  • [40] Targeting Specificity of Cas9 Nucleases and Tools for the Selection of High Fidelity Cas9 Targets
    Scott, David A.
    Hsu, Patrick D.
    Weinstein, Joshua A.
    Holmes, Benjamin
    Ran, F. Ann
    Zhang, Feng
    MOLECULAR THERAPY, 2014, 22 : S125 - S126
12345