Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS

被引：3

作者：

Robinson, Emma ^{[1
]}

Shorter, James ^{[2
]}

Guo, Lin ^{[1
]}

机构：

[1] Jefferson Univ, Dept Biochem & Mol Biol, Philadelphia, PA 19107 USA

[2] Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

BIO-PROTOCOL | 2020年 / 10卷 / 16期

关键词：

FUS; Liquid-liquid phase separation; Nuclear import receptor; Kap beta 2; Protein aggregation; NUCLEAR IMPORT RECEPTOR; RNA-BINDING PROTEINS; TRANSITION; DROPLETS; DOMAINS; TDP-43;

D O I：

10.21769/BioProtoc.3725

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kap beta 2 and testing the ability of Kap beta 2 to mitigate FUS aggregation and liquid-liquid phase separation.

引用

页数：13

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