Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS

被引：3

作者：

Robinson, Emma ^{[1
]}

Shorter, James ^{[2
]}

Guo, Lin ^{[1
]}

机构：

[1] Jefferson Univ, Dept Biochem & Mol Biol, Philadelphia, PA 19107 USA

[2] Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

BIO-PROTOCOL | 2020年 / 10卷 / 16期

关键词：

FUS; Liquid-liquid phase separation; Nuclear import receptor; Kap beta 2; Protein aggregation; NUCLEAR IMPORT RECEPTOR; RNA-BINDING PROTEINS; TRANSITION; DROPLETS; DOMAINS; TDP-43;

D O I：

10.21769/BioProtoc.3725

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kap beta 2 and testing the ability of Kap beta 2 to mitigate FUS aggregation and liquid-liquid phase separation.

引用

页数：13

共 50 条

[21] The disordered protein SERF promotes α-Synuclein aggregation through liquid-liquid phase separation
Liu, He-Ning
Wang, Ting
Hu, Jin-Jian
Chen, Long
Shi, Xiangyan
Li, Yan-Mei
Luo, Shi-Zhong
JOURNAL OF BIOLOGICAL CHEMISTRY, 2024, 300 (03)
[22] Hsp70 exhibits a liquid-liquid phase separation ability and chaperones condensed FUS against amyloid aggregation
Li, Yichen
Gu, Jinge
Wang, Chen
Hu, Jiaojiao
Zhang, Shenqing
Liu, Cong
Zhang, Shengnan
Fang, Yanshan
Li, Dan
ISCIENCE, 2022, 25 (06)
[23] Insights into the Atomistic Mechanisms of Phosphorylation in Disrupting Liquid-Liquid Phase Separation and Aggregation of the FUS Low-Complexity Domain
Lao, Zenghui
Dong, Xuewei
Liu, Xianshi
Li, Fangying
Chen, Yujie
Tang, Yiming
Wei, Guanghong
Journal of Chemical Information and Modeling, 2022, 62 (13) : 3227 - 3238
[24] α-Synuclein aggregation nucleates through liquid-liquid phase separation
Ray, Soumik
Singh, Nitu
Kumar, Rakesh
Patel, Komal
Pandey, Satyaprakash
Datta, Debalina
Mahato, Jaladhar
Panigrahi, Rajlaxmi
Navalkar, Ambuja
Mehra, Surabhi
Gadhe, Laxmikant
Chatterjee, Debdeep
Sawner, Ajay Singh
Maiti, Siddhartha
Bhatia, Sandhya
Gerez, Juan Atilio
Chowdhury, Arindam
Kumar, Ashutosh
Padinhateeri, Ranjith
Riek, Roland
Krishnamoorthy, G.
Maji, Samir K.
NATURE CHEMISTRY, 2020, 12 (08) : 705 - 716
[25] Amyloid Aggregation under the Lens of Liquid-Liquid Phase Separation
Xing, Yanting
Nandakumar, Aparna
Kakinen, Aleksandr
Sun, Yunxiang
Davis, Thomas P.
Ke, Pu Chun
Ding, Feng
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2021, 12 (01): : 368 - 378
[26] ALS-linked FUS mutations dysregulate G-quadruplex-dependent liquid-liquid phase separation and liquid-to-solid transition
Ishiguro, Akira
Lu, Jun
Ozawa, Daisaku
Nagai, Yoshitaka
Ishihama, Akira
JOURNAL OF BIOLOGICAL CHEMISTRY, 2021, 297 (05)
[27] The phase separation-dependent FUS interactome reveals nuclear and cytoplasmic function of liquid-liquid phase separation
Reber, Stefan
Jutzi, Daniel
Lindsay, Helen
Devoy, Anny
Mechtersheimer, Jonas
Levone, Brunno Rocha
Domanski, Michal
Bentmann, Eva
Dormann, Dorothee
Muehlemann, Oliver
Barabino, Silvia M. L.
Ruepp, Marc-David
NUCLEIC ACIDS RESEARCH, 2021, 49 (13) : 7713 - 7731
[28] FUS-dependent liquid-liquid phase separation is important for DNA repair initiation
Levone, Brunno R.
Lenzken, Silvia C.
Antonaci, Marco
Maiser, Andreas
Rapp, Alexander
Conte, Francesca
Reber, Stefan
Mechtersheimer, Jonas
Ronchi, Antonella E.
Muehlemann, Oliver
Leonhardt, Heinrich
Cardoso, M. Cristina
Ruepp, Marc-David
Barabino, Silvia M. L.
JOURNAL OF CELL BIOLOGY, 2021, 220 (05):
[29] Mimicking hypomethylation of FUS requires liquid-liquid phase separation to induce synaptic dysfunctions
Kim, Seung Chan
Mitchell, Scott J.
Qamar, Seema
Whitcomb, Daniel J.
Ruepp, Marc-David
St George-Hyslop, Peter
Cho, Kwangwook
ACTA NEUROPATHOLOGICA COMMUNICATIONS, 2023, 11 (01)
[30] Protein Databases Related to Liquid-Liquid Phase Separation
Li, Qian
Wang, Xi
Dou, Zhihui
Yang, Weishan
Huang, Beifang
Lou, Jizhong
Zhang, Zhuqing
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (18) : 1 - 16

← 1 2 3 4 5 →