A dual-response ratiometric fluorescent probe for hypochlorite and hydrazine detection and its imaging in living cells

被引：37

作者：

Wang, Lin ^{[1
]}

Pan, Qi ^{[2
]}

Chen, Yuan ^{[2
]}

Ou, Yunfu ^{[1
]}

Li, Huanyong ^{[1
]}

Li, Bowen ^{[2
]}

机构：

[1] Jinan Univ, Analyt & Testing Ctr, Guangzhou 510632, Peoples R China

[2] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2020年 / 241卷 / 241期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Ratiometric fluorescent; Carbazole; Hypochlorite detection; Hydrazine detection; Cell imaging; TURN-ON; SELECTIVE VISUALIZATION; MITOCHONDRIA; CHEMOSENSOR; ACID; AL3+; RECOGNITION;

D O I：

10.1016/j.saa.2020.118672

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this work, a dual-response ratiometric fluorescent probe (E)-3-(5-(2-nitrovinyl)thiophen-2-yl)-9-phenyl-9H-carbazole (NTPC) for high selectivity and sensitivity detection of ClO- and N2H4 was successfully developed. This probe NTPC showed ratiometric fluorescent response to ClO- and N2H4, which induces obvious naked-eye color changes, respectively. In addition, the NTPC for ClO- and N2H4 detection displayed low detection limits of 71.4 nM and 0.6 mu M, respectively. And the sensing mechanism of NTPC with ClO- and N2H4 was well confirmed by H-1 NMR and HR-MS spectra. Moreover, this novel probe was applied to monitoring and differentiating ClO- and N2H4 in living cells, and exhibits good biocompatibility and low cytotoxicity. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：7

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