Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human CAD Protein

被引：2
|
作者：
Li, Mengbin [1 ]
Zhang, Hongwei [1 ]
Hong, Liu [1 ]
Jin, Yan [1 ]
Gong, Taiqian [1 ]
Sun, Li [1 ]
Yang, Jianjun [1 ]
Zheng, Xiushan [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Prov, Peoples R China
来源：
HYBRIDOMA | 2009年 / 28卷 / 02期
基金：
中国国家自然科学基金;
关键词：
ACONITIC ACID DECARBOXYLASE; CCAAT-BINDING-FACTOR; ASPERGILLUS-TERREUS; CELL-PROLIFERATION; ITACONIC ACID; CANCER CELLS; ZNRD1; GENE; ZINC-RIBBON-DOMAIN-CONTAINING-1; BIOSYNTHESIS; ARREST;
D O I：
10.1089/hyb.2008.0091
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have efficiently generated the first monoclonal antibody against the cis-aconitic acid decarboxylase (CAD) protein, which is an enzyme of low stability. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-CAD fusion protein or purified 6 x His-ZNRD1 fusion protein as a control. One monoclonal antibody (MAb) named Z12 (IgG1), which is effective in detecting the recombinant and the cellular protein, was characterized by ELISA and Western immunoblotting. The CAD protein was also detected in gastric cancer and colon cancer tissues by immunohistochemical analysis. Thus, Z12 binds to native CAD protein and should be useful in studies of CAD protein function and expression.
引用
收藏
页码：129 / 132
页数：4
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