Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human CAD Protein

被引：2
|
作者：
Li, Mengbin [1 ]
Zhang, Hongwei [1 ]
Hong, Liu [1 ]
Jin, Yan [1 ]
Gong, Taiqian [1 ]
Sun, Li [1 ]
Yang, Jianjun [1 ]
Zheng, Xiushan [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Prov, Peoples R China
来源：
HYBRIDOMA | 2009年 / 28卷 / 02期
基金：
中国国家自然科学基金;
关键词：
ACONITIC ACID DECARBOXYLASE; CCAAT-BINDING-FACTOR; ASPERGILLUS-TERREUS; CELL-PROLIFERATION; ITACONIC ACID; CANCER CELLS; ZNRD1; GENE; ZINC-RIBBON-DOMAIN-CONTAINING-1; BIOSYNTHESIS; ARREST;
D O I：
10.1089/hyb.2008.0091
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have efficiently generated the first monoclonal antibody against the cis-aconitic acid decarboxylase (CAD) protein, which is an enzyme of low stability. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-CAD fusion protein or purified 6 x His-ZNRD1 fusion protein as a control. One monoclonal antibody (MAb) named Z12 (IgG1), which is effective in detecting the recombinant and the cellular protein, was characterized by ELISA and Western immunoblotting. The CAD protein was also detected in gastric cancer and colon cancer tissues by immunohistochemical analysis. Thus, Z12 binds to native CAD protein and should be useful in studies of CAD protein function and expression.
引用
收藏
页码：129 / 132
页数：4
相关论文
共 50 条
  • [11] Preparation and characterization of a monoclonal antibody against the protein LIGHT
    Fan, KX
    Zhou, Q
    Wang, H
    Guo, YJ
    HYBRIDOMA, 2005, 24 (06): : 309 - 313
  • [12] PREPARATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY TO HUMAN SULFOMUCIN
    MILTON, JD
    ECCLESTON, D
    PARKER, N
    TSAI, HH
    RYDER, SD
    COXSINGH, J
    RHODES, JM
    GUT, 1990, 31 (10) : A1179 - A1179
  • [13] Characterization of golimumab (CNTO 148), a novel fully human monoclonal antibody specific for human TNFalpha
    Shealy, D.
    Cai, A.
    Lacy, E.
    Nesspor, T.
    Staquet, K.
    Johns, L.
    Baker, A.
    Brigham-Burke, M.
    Emmell, E.
    Bugelski, P.
    Verzaal, P.
    Nagelkerken, L.
    Scallon, B.
    Giles-Komar, J.
    ANNALS OF THE RHEUMATIC DISEASES, 2007, 66 : 151 - 151
  • [14] Preparation of a Novel Monoclonal Antibody Specific to DARPP-32
    Yang, Jianjun
    Li, Mengbin
    Hong, Liu
    Zhang, Hongwei
    Sun, Li
    Wang, Weizhong
    HYBRIDOMA, 2010, 29 (04): : 351 - 354
  • [15] A Monoclonal Antibody Specific to Human Myxovirus Resistance Protein A
    Wu, Kang
    Liu, Peng
    Guo, Bingnan
    Zhang, Yinsheng
    Zhao, Lixiang
    Yang, Jicheng
    Liu, Haiyan
    HYBRIDOMA, 2010, 29 (01): : 31 - 36
  • [16] Preparation and application of a novel monoclonal antibody specific for the heat shock protein 60 of Lawsonia intracellularis
    XIAO Ning
    Lü Yun-yun
    LI Jian-nan
    CHEN Chang-feng
    LIN Hui-xing
    FAN Hong-jie
    JournalofIntegrativeAgriculture, 2023, 22 (09) : 2824 - 2833
  • [17] Preparation and application of a novel monoclonal antibody specific for the heat shock protein 60 of Lawsonia intracellularis
    Xiao, Ning
    Lu, Yun-yun
    Li, Jian-nan
    Chen, Chang-feng
    Lin, Hui-xing
    Fan, Hong-jie
    JOURNAL OF INTEGRATIVE AGRICULTURE, 2023, 22 (09) : 2824 - 2833
  • [18] GENERATION AND CHARACTERIZATION OF A NOVEL MONOCLONAL-ANTIBODY SPECIFIC FOR BOTH MURINE AND HUMAN KERATINOCYTES
    MOHAMADZADEH, M
    KOLDE, G
    ENK, A
    SALOGA, J
    KNOP, J
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (04) : 564 - 564
  • [19] Preparation and application of a novel monoclonal antibody specific for human B7-H3
    Shi, Jian
    Zhang, Dong-Lei
    Cui, Zhi-Chu
    Wang, Hui-Min
    MOLECULAR MEDICINE REPORTS, 2016, 14 (01) : 943 - 948
  • [20] A Novel Monoclonal Antibody Specific to DIXDC1 Protein
    Liu, Tantan
    Wang, Lei
    Chen, Qi
    Zheng, Li
    Zhu, Hongguang
    HYBRIDOMA, 2009, 28 (03): : 183 - 188
12345