Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human CAD Protein

被引：2

作者：

Li, Mengbin ^{[1
]}

Zhang, Hongwei ^{[1
]}

Hong, Liu ^{[1
]}

Jin, Yan ^{[1
]}

Gong, Taiqian ^{[1
]}

Sun, Li ^{[1
]}

Yang, Jianjun ^{[1
]}

Zheng, Xiushan ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Prov, Peoples R China

来源：

HYBRIDOMA | 2009年 / 28卷 / 02期

基金：

中国国家自然科学基金;

关键词：

ACONITIC ACID DECARBOXYLASE; CCAAT-BINDING-FACTOR; ASPERGILLUS-TERREUS; CELL-PROLIFERATION; ITACONIC ACID; CANCER CELLS; ZNRD1; GENE; ZINC-RIBBON-DOMAIN-CONTAINING-1; BIOSYNTHESIS; ARREST;

D O I：

10.1089/hyb.2008.0091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have efficiently generated the first monoclonal antibody against the cis-aconitic acid decarboxylase (CAD) protein, which is an enzyme of low stability. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-CAD fusion protein or purified 6 x His-ZNRD1 fusion protein as a control. One monoclonal antibody (MAb) named Z12 (IgG1), which is effective in detecting the recombinant and the cellular protein, was characterized by ELISA and Western immunoblotting. The CAD protein was also detected in gastric cancer and colon cancer tissues by immunohistochemical analysis. Thus, Z12 binds to native CAD protein and should be useful in studies of CAD protein function and expression.

引用

页码：129 / 132

页数：4

共 50 条

[1] Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein
Cao, Jun
HYBRIDOMA, 2007, 26 (06): : 417 - 421
[2] Preparation and characterization of a novel monoclonal antibody specific to human NOB1 protein
Han, Yu
Hong, Liu
Qiu, Jianhua
Qiao, L.
Zhong, Cuiping
Xue, Tao
Wang, Menghang
HYBRIDOMA, 2008, 27 (03): : 187 - 190
[3] Preparation and characterization of a novel monoclonal antibody specific to human ZNRD1 protein
Hong, L
Zhang, YM
Han, S
Wang, J
Shi, YQ
Pan, YL
Liu, N
Zhang, XY
Fan, DM
HYBRIDOMA AND HYBRIDOMICS, 2004, 23 (01): : 65 - 68
[4] Preparation and Characterization of a Novel Monoclonal Antibody Specific to Peptide VEHVVADAGAFLRH
Cao, Jun
HYBRIDOMA, 2009, 28 (01): : 59 - 62
[5] Preparation and characterization of a monoclonal antibody specific to Plasmodium falciparum TATA binding protein
Ruvalcaba-Salazar, Omar-Kayyhan
Romero-Ramirez, Hector
Santos-Argumedo, Leopoldo
Vargas, Miguel
Hernandez-Rivas, Rosaura
HYBRIDOMA, 2006, 25 (06): : 367 - 371
[6] Preparation of a monoclonal antibody specific for human stanniocalcin
Koide, Y
Igarashi, A
Sakuma, N
Sato, K
Shibata, S
Tada, K
Takano, S
Sasaki, T
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1998, 21 (12) : 1352 - 1355
[7] Characterization of a novel human BFL-1-specific monoclonal antibody
Lahiru Gangoda
Charis E. Teh
Michael A. Dengler
Sarah A. Best
Clare E. Weeden
Lin Tai
Erinna F. Lee
Walter D. Fairlie
Kate D. Sutherland
Leonard C. Harrison
Daniel H. Gray
Andreas Strasser
Marco J. Herold
Cell Death & Differentiation, 2020, 27 : 826 - 828
[8] Characterization of a novel human BFL-1-specific monoclonal antibody
Gangoda, Lahiru
Teh, Charis E.
Dengler, Michael A.
Best, Sarah A.
Weeden, Clare E.
Tai, Lin
Lee, Erinna F.
Fairlie, Walter D.
Sutherland, Kate D.
Harrison, Leonard C.
Gray, Daniel H.
Strasser, Andreas
Herold, Marco J.
CELL DEATH AND DIFFERENTIATION, 2020, 27 (02): : 826 - 828
[9] Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid protein
Shin, Gu-Choul
Chung, Yoon-Seok
Kim, In-Soo
Cho, Hae-Wol
Kang, Chun
VIRUS RESEARCH, 2006, 122 (1-2) : 109 - 118
[10] Preparation and characterization of a fully human monoclonal antibody specific for human tumor necrosis factor alpha
Liao, Xinmei
Liang, Hui
Pan, Jian
Zhang, Qian
Niu, Jiaqi
Xue, Cuili
Ni, Jian
Cui, Daxiang
BIOENGINEERED, 2021, 12 (02) : 10821 - 10834

← 1 2 3 4 5 →