Direct identification of mycobacteria from smear-positive samples using real-time polymerase chain reaction

被引：3
|
作者：
Marekovic, I. [1 ,2 ]
Bosnjak, Z. [2 ]
Plecko, V. [1 ,2 ]
机构：
[1] Univ Zagreb, Sch Med, Zagreb 41001, Croatia
[2] Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb 10000, Croatia
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 08期
关键词：
acid-fast bacilli grade; bronchoalveolar lavage; tuberculosis; SPUTUM SAMPLES; PCR; TUBERCULOSIS;
D O I：
10.5588/ijtld.13.0042
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of the present study was to investigate whether real-time polymerase chain reaction (PCR) has a low identification rate for samples with low acid-fast bacilli (AFB) grades, including those obtained using bronchoscopy. When 50 smear-positive samples were compared by AFB score and PCR result, PCR was 100% successful in identifying AFB 2+ and AFB 3+ samples. However, only 14 of 26(52%) AFB 1+ samples were identified. In paucibacillary smear-positive samples, PCR is not reliable enough to exclude tuberculosis, but in smear-positive patients with high AFB grades PCR can immediately change the clinical management of the disease.
引用
收藏
页码：978 / 980
页数：3
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