Direct identification of mycobacteria from smear-positive samples using real-time polymerase chain reaction

被引：3
|
作者：
Marekovic, I. [1 ,2 ]
Bosnjak, Z. [2 ]
Plecko, V. [1 ,2 ]
机构：
[1] Univ Zagreb, Sch Med, Zagreb 41001, Croatia
[2] Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb 10000, Croatia
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 08期
关键词：
acid-fast bacilli grade; bronchoalveolar lavage; tuberculosis; SPUTUM SAMPLES; PCR; TUBERCULOSIS;
D O I：
10.5588/ijtld.13.0042
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of the present study was to investigate whether real-time polymerase chain reaction (PCR) has a low identification rate for samples with low acid-fast bacilli (AFB) grades, including those obtained using bronchoscopy. When 50 smear-positive samples were compared by AFB score and PCR result, PCR was 100% successful in identifying AFB 2+ and AFB 3+ samples. However, only 14 of 26(52%) AFB 1+ samples were identified. In paucibacillary smear-positive samples, PCR is not reliable enough to exclude tuberculosis, but in smear-positive patients with high AFB grades PCR can immediately change the clinical management of the disease.
引用
收藏
页码：978 / 980
页数：3
相关论文
共 50 条
  • [21] Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples
    Tong Zhang
    Herbert H. P. Fang
    Applied Microbiology and Biotechnology, 2006, 70 : 281 - 289
  • [22] The utility of real-time polymerase chain reaction for molecular studies on faecal samples
    Morf, N.
    Morrogh-Bernard, H.
    Kruetzen, M.
    FOLIA PRIMATOLOGICA, 2008, 79 (05) : 362 - 362
  • [23] Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction
    Perotti, Mario
    Mancini, Nicasio
    Cavallero, Annalisa
    Carletti, Silvia
    Canducci, Filippo
    Burioni, Roberto
    Clementi, Massimo
    JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 65 (03) : 632 - 636
  • [24] Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples
    Zhang, T
    Fang, HHP
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 70 (03) : 281 - 289
  • [25] Comparison Of Real-Time Polymerase Chain Reaction And Conventional Smear Methods For The Detection Of Mycobacterium Tuberculosis In Sputum Samples In South Korea
    Choi, S. M.
    Park, J. S.
    Lee, S. W.
    Yoon, H. I.
    Lee, C. -T.
    Lee, J. H.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2011, 183
  • [26] Electrochemical real-time polymerase chain reaction
    Yeung, Stephen S. W.
    Lee, Thomas M. H.
    Hsing, I-Ming
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (41) : 13374 - 13375
  • [27] Electrochemical real-time polymerase chain reaction
    Yeung, Stephen S. W.
    Lee, Thomas M. H.
    Hsing, I.-Ming
    Journal of the American Chemical Society, 2006, 128 (41): : 13374 - 13375
  • [28] Direct Quantification and Distribution of Tetracycline-Resistant Genes in Meat Samples by Real-Time Polymerase Chain Reaction
    Guarddon, Monica
    Miranda, Jose M.
    Vazquez, Beatriz I.
    Cepeda, Alberto
    Franco, Carlos M.
    JOURNAL OF FOOD SCIENCE, 2012, 77 (07) : M372 - M376
  • [29] Comparison of real-time polymerase chain reaction and conventional polymerase chain reaction methods for the rapid identification of Bacillus anthracis
    Kenar, Levent
    Ortatatli, Mesut
    Karayilanoglu, Turan
    Yaren, Hakan
    Sen, Selahattin
    MILITARY MEDICINE, 2007, 172 (07) : 773 - 776
  • [30] Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples
    Ramamurthy, M.
    Alexander, M.
    Aaron, S.
    Kannangai, R.
    Ravi, V.
    Sridharan, G.
    Abraham, A. M.
    INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 29 (02) : 102 - 109
12345