Key Rep-Proteins Necessary for the Adeno-Associated Virus Production by Transient Transfection in HEK293 Cells in Suspension and Serum-Free Medium

被引：0
|
作者：
Chahal, Parminder [1 ]
Elahi, Mehdy [1 ]
O'Neil, Patrick. [1 ]
Venne, Marie-Helene. [1 ]
Nazemi-Moghaddam, Nazila [1 ]
Gilbert, Renald [1 ]
机构：
[1] Natl Res Council Canada, Human Hlth Therapeut Portfolio, Montreal, PQ, Canada
来源：
MOLECULAR THERAPY | 2018年 / 26卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
713
引用
收藏
页码：328 / 328
页数：1
相关论文
共 50 条
  • [31] High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells
    Markus Hildinger
    Lucia Baldi
    Matthieu Stettler
    Florian M. Wurm
    Biotechnology Letters, 2007, 29 : 1713 - 1721
  • [32] Production of Recombinant Adeno-Associated Virus Through High-Cell-Density Transfection of HEK293 Cells Based on Fed-Perfusion Culture
    Deng, Zhe
    Lv, Yan-Ling
    Wang, Xin-Tao
    Yuan, Long-Hui
    Zhao, Kai
    Du, Zeng-Min
    Xiao, Xiao
    HUMAN GENE THERAPY, 2025,
  • [33] Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
    Pietersz, Kimberly L.
    Nijhuis, Paul J. H.
    Klunder, Matthijs H. M.
    van den Herik, Joelle
    Hobo, Barbara
    de Winter, Fred
    Verhaagen, Joost
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2024, (206):
  • [34] Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency
    Pham, PL
    Perret, S
    Doan, HC
    Cass, B
    St-Laurent, G
    Kamen, A
    Durocher, Y
    BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (03) : 332 - 342
  • [35] Evaluation of a new chemically defined medium for production of AAV vectors by transient transfection of suspension HEK293 cells in stirred tank bioreactors
    Guianvarch, L.
    Boussicault, F.
    Dufour, D.
    Oubaidour, M. A.
    Giambelluco, E.
    Sirantoine, G.
    Hebben, M.
    HUMAN GENE THERAPY, 2016, 27 (11) : A166 - A166
  • [36] Improving the expression yield of recombinant adeno-associated virus serotype 2 using dimethyl sulfoxide DMSO as an additive to the triple transient transfection process of HEK293 cells
    Burns, Alexander
    Ramos-Sono, Daniel
    Datta, Saurav
    BIOTECHNOLOGY PROGRESS, 2025,
  • [37] Use of Dynamic Light Scattering to Optimize Scale-able Transfection Complex Formation for AAV Production in Serum-Free HEK293 Suspension Cultures
    Beckett, Rebecca L.
    Bucan, Casey
    Rossi, Nicolas A. A.
    Ludtke, James
    Juckem, Laura K.
    Bhatia, Neehar
    Ross, Carl A.
    MOLECULAR THERAPY, 2017, 25 (05) : 226 - 226
  • [38] Serum-free production and column purification of adeno-associated virus type 5
    Smith, RH
    Ding, CT
    Kotin, RM
    JOURNAL OF VIROLOGICAL METHODS, 2003, 114 (02) : 115 - 124
  • [39] Development of a Scalable Adeno Associated Virus Production Process by Transient Transfection in Suspension Cells
    Magnusson, Ann-Christin
    Blanck, Eva
    Thelander, Josefin
    Larsson, Albin
    Persson, Sebastian
    Bjorklund, Viktor
    Monie, Elin
    Areskoug, Daphne
    Lundgren, Mats
    MOLECULAR THERAPY, 2021, 29 (04) : 399 - 399
  • [40] The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles
    Jang, Mi
    Pete, Ellen Sofie
    Bruheim, Per
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
12345