Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater

被引：30
|
作者：
Dumke, Roger [1 ]
de la Cruz Barron, Magali [2 ,3 ]
Oertel, Reinhard [4 ]
Helm, Bjoern [5 ]
Kallies, Rene [6 ]
Berendonk, Thomas U. [2 ]
Dalpke, Alexander [1 ]
机构：
[1] Tech Univ Dresden, Inst Med Microbiol & Virol, Fetscherstr 74, D-01307 Dresden, Germany
[2] Tech Univ Dresden, Inst Hydrobiol, Helmholtzstr 10, D-01069 Dresden, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept River Ecol, Bruckstr 3a, D-39114 Magdeburg, Germany
[4] Tech Univ Dresden, Inst Clin Pharmacol, Fetscherstr 74, D-01307 Dresden, Germany
[5] Tech Univ Dresden, Inst Urban & Ind Water Management, Helmholtzstr 10, D-01069 Dresden, Germany
[6] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany
来源：
PATHOGENS | 2021年 / 10卷 / 02期
关键词：
SARS-CoV-2; wastewater; virus concentration; wastewater-based epidemiology; monitoring; surveillance; VIRUS; SURVEILLANCE;
D O I：
10.3390/pathogens10020195
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
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收藏
页码：1 / 7
页数：7
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