Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater

被引：30

作者：

Dumke, Roger ^{[1
]}

de la Cruz Barron, Magali ^{[2
,3
]}

Oertel, Reinhard ^{[4
]}

Helm, Bjoern ^{[5
]}

Kallies, Rene ^{[6
]}

Berendonk, Thomas U. ^{[2
]}

Dalpke, Alexander ^{[1
]}

机构：

[1] Tech Univ Dresden, Inst Med Microbiol & Virol, Fetscherstr 74, D-01307 Dresden, Germany

[2] Tech Univ Dresden, Inst Hydrobiol, Helmholtzstr 10, D-01069 Dresden, Germany

[3] UFZ Helmholtz Ctr Environm Res, Dept River Ecol, Bruckstr 3a, D-39114 Magdeburg, Germany

[4] Tech Univ Dresden, Inst Clin Pharmacol, Fetscherstr 74, D-01307 Dresden, Germany

[5] Tech Univ Dresden, Inst Urban & Ind Water Management, Helmholtzstr 10, D-01069 Dresden, Germany

[6] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany

来源：

PATHOGENS | 2021年 / 10卷 / 02期

关键词：

SARS-CoV-2; wastewater; virus concentration; wastewater-based epidemiology; monitoring; surveillance; VIRUS; SURVEILLANCE;

D O I：

10.3390/pathogens10020195

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.

引用

页码：1 / 7

页数：7

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