Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays

被引：16
|
作者：
Schaller, Vincent [1 ]
Sanz-Velasco, Anke [1 ]
Kalabukhov, Alexey [1 ]
Schneiderman, Justin F. [1 ]
Oisjoen, Fredrik [1 ]
Jesorka, Aldo [2 ]
Astalan, Andrea Prieto [3 ]
Krozer, Anatol [3 ]
Rusu, Cristina [3 ]
Enoksson, Peter [1 ]
Winkler, Dag [1 ]
机构：
[1] Chalmers, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden
[2] Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden
[3] Imego Inst, SE-40014 Gothenburg, Sweden
来源：
LAB ON A CHIP | 2009年 / 9卷 / 23期
关键词：
ON-A-CHIP; LIQUID DROPLETS; NANOPARTICLES; ACTUATION; PARTICLES;
D O I：
10.1039/b912646e
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 mu l de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 ml sample droplet compared to a 30 ml sample volume.
引用
收藏
页码：3433 / 3436
页数：4
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