An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene

被引：6

作者：

Gibb, Ewan A. ^{[1
]}

Edgell, David R. ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

来源：

JOURNAL OF BACTERIOLOGY | 2009年 / 191卷 / 07期

关键词：

GROUP-I INTRONS; MARKER EXCLUSION; EXPRESSION; REDUCTASE; PROTEINS; HOMOLOGY; SEQUENCE;

D O I：

10.1128/JB.01751-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced beta-galactosidase activity, consistent with translational regulation.

引用

页码：2409 / 2413

页数：5

共 50 条

[31] SITE-DIRECTED GENERATION OF NUCLEOTIDE SUBSTITUTIONS IN COAT CISTRON RIBOSOME BINDING-SITE OF Q-BETA-RNA
TANIGUCHI, T
BILLETER, MA
WEISSMANN, C
EXPERIENTIA, 1975, 31 (06): : 749 - 749
[32] INCREASED EXPRESSION OF A CLONED GENE BY LOCAL MUTAGENESIS OF ITS PROMOTER AND RIBOSOME BINDING-SITE
WARBURTON, N
BOSELEY, PG
PORTER, AG
NUCLEIC ACIDS RESEARCH, 1983, 11 (17) : 5837 - 5854
[33] THE WHEAT MITOCHONDRIAL GENE FOR APOCYTOCHROME-B - ABSENCE OF A PROKARYOTIC RIBOSOME BINDING-SITE
BOER, PH
MCINTOSH, JE
GRAY, MW
BONEN, L
NUCLEIC ACIDS RESEARCH, 1985, 13 (07) : 2281 - 2292
[34] A GROUP-I INTRON IN THE CHLOROPLAST LARGE SUBUNIT RIBOSOMAL-RNA GENE OF CHLAMYDOMONAS-EUGAMETOS ENCODES A DOUBLE-STRAND ENDONUCLEASE THAT CLEAVES THE HOMING SITE OF THIS INTRON
GAUTHIER, A
TURMEL, M
LEMIEUX, C
CURRENT GENETICS, 1991, 19 (01) : 43 - 47
[35] RIBOSOMAL-RNA AND THE SITE SPECIFICITY OF CHLORAMPHENICOL-DEPENDENT RIBOSOME STALLING IN CAT GENE LEADERS
LOVETT, PS
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 646 : 31 - 34
[36] A RIBOSOME BINDING-SITE SEQUENCE IS NECESSARY FOR EFFICIENT EXPRESSION OF THE DISTAL GENE OF A TRANSLATIONALLY-COUPLED GENE PAIR
DAS, A
YANOFSKY, C
NUCLEIC ACIDS RESEARCH, 1984, 12 (11) : 4757 - 4768
[37] THE MESSENGER-RNA FOR THE 23S RIBOSOMAL-RNA METHYLASE ENCODED BY THE ERME GENE OF SACCHAROPOLYSPORA-ERYTHRAEA IS TRANSLATED IN THE ABSENCE OF A CONVENTIONAL RIBOSOME-BINDING SITE
BIBB, MJ
WHITE, J
WARD, JM
JANSSEN, GR
MOLECULAR MICROBIOLOGY, 1994, 14 (03) : 533 - 545
[38] PULLING THE RIBOSOME OUT OF FRAME BY +1 AT A PROGRAMMED FRAMESHIFT SITE BY COGNATE BINDING OF AMINOACYL-TRANSFER-RNA
PANDE, S
VIMALADITHAN, A
ZHAO, H
FARABAUGH, PJ
MOLECULAR AND CELLULAR BIOLOGY, 1995, 15 (01) : 298 - 304
[39] A 2ND PUTATIVE MESSENGER-RNA BINDING-SITE ON THE ESCHERICHIA-COLI RIBOSOME
IVANOV, IG
ALEXANDROVA, RA
DRAGULEV, BP
ABOUHAIDAR, MG
GENE, 1995, 160 (01) : 75 - 79
[40] IDENTIFICATION OF A RIBOSOME-BINDING SITE FOR A LEADER PEPTIDE ENCODED BY ROUS-SARCOMA VIRUS-RNA
PETERSEN, RB
HENSEL, CH
HACKETT, PB
JOURNAL OF VIROLOGY, 1984, 51 (03) : 722 - 729

← 1 2 3 4 5 →