An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene

被引：6
|
作者：
Gibb, Ewan A. [1 ]
Edgell, David R. [1 ]
机构：
[1] Univ Western Ontario, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada
来源：
JOURNAL OF BACTERIOLOGY | 2009年 / 191卷 / 07期
关键词：
GROUP-I INTRONS; MARKER EXCLUSION; EXPRESSION; REDUCTASE; PROTEINS; HOMOLOGY; SEQUENCE;
D O I：
10.1128/JB.01751-08
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced beta-galactosidase activity, consistent with translational regulation.
引用
收藏
页码：2409 / 2413
页数：5
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