A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

被引：12
|
作者：
Dewi, Chitra Umala [1 ]
Mason, Michele [1 ]
Cohen-Hyams, Tzipi [2 ]
Killingsworth, Murray C. [2 ]
Harman, David G. [1 ]
Gnanasambandapillai, Vikkitharan [3 ]
Liyanage, Liwan [4 ]
O'Connor, Michael D. [1 ]
机构：
[1] Western Sydney Univ, Sch Med, Campbelltown, NSW 2560, Australia
[2] Ingham Inst, NSW Hlth Pathol & Correlat Microscopy Facil, Electron Microscopy Lab, Liverpool, NSW 2170, Australia
[3] Garvan Weizmann Ctr Cellular Genom, Darlinghurst, NSW 2010, Australia
[4] Western Sydney Univ, Sch Comp Data & Math Sci, Campbelltown, NSW 2560, Australia
来源：
EXPERIMENTAL EYE RESEARCH | 2021年 / 202卷
关键词：
Human; Lens; Lens epithelial cell; ROR1; Pluripotent stem cell; Micro-lens; Cataract; Single cell RNA-Sequencing; Bioinformatics;
D O I：
10.1016/j.exer.2020.108317
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of lightfocusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.
引用
收藏
页数：9
相关论文
共 50 条
  • [41] Robust and Highly Specific Generation of Human Airway Epithelial Basal Stem Cells from Induced Pluripotent Stem Cells
    Li, Y.
    Goldfarbmuren, K. C.
    Rios, C.
    Seibold, M. A.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2020, 201
  • [42] Differentiation of haemopoietic stem cells and progenitor cells from human pluripotent stem cells
    Easterbrook, Jennifer
    Forrester, Lesley
    Medvinsky, Alexander
    LANCET, 2016, 387 : 39 - 39
  • [43] Induction of Human Trophoblast Stem Cells from Somatic Cells and Pluripotent Stem Cells
    Castel, Gael
    Meistermann, Dimitri
    Bretin, Betty
    Firmin, Julie
    Blin, Justine
    Loubersac, Sophie
    Bruneau, Alexandre
    Chevolleau, Simon
    Kilens, Stephanie
    Chariau, Caroline
    Gaignerie, Anne
    Francheteau, Quentin
    Kagawa, Harunobu
    Charpentier, Eric
    Flippe, Lea
    Francois--Campion, Valentin
    Haider, Sandra
    Dietrich, Bianca
    Knoefler, Martin
    Arima, Takahiro
    Bourdon, Jeremie
    Rivron, Nicolas
    Masson, Damien
    Fournier, Thierry
    Okae, Hiroaki
    Freour, Thomas
    David, Laurent
    CELL REPORTS, 2020, 33 (08):
  • [44] NK Cells from Human Pluripotent Stem Cells for Immunotherapy
    Maysubara, Hiroyuki
    Niwa, Akira
    Nakahata, Tatsutoshi
    Saito, Megumu K.
    BLOOD, 2018, 132
  • [45] Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells
    Liu, Haikun
    Guo, Dongsheng
    Ruzi, Aynisahan
    Chen, Yan
    Pan, Tingcai
    Yang, Fan
    Li, Jialiang
    Xu, Kecheng
    Zhou, Tiancheng
    Qin, Dajiang
    Li, Yin-xiong
    PLOS ONE, 2017, 12 (06):
  • [46] Derivation of trophoblast stem cells from naive human pluripotent stem cells
    Dong, Chen
    Beltcheva, Mariana
    Gontarz, Paul
    Zhang, Bo
    Popli, Pooja
    Fischer, Laura A.
    Khan, Shafqat A.
    Park, Kyoung-mi
    Yoon, Eun-Ja
    Xing, Xiaoyun
    Kommagani, Ramakrishna
    Wang, Ting
    Solnica-Krezel, Lilianna
    Theunissen, Thorold W.
    ELIFE, 2020, 9
  • [47] Development of Hematopoietic Stem and Progenitor Cells From Human Pluripotent Stem Cells
    Chen, Tong
    Wang, Fen
    Wu, Mengyao
    Wang, Zack Z.
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2015, 116 (07) : 1179 - 1189
  • [48] Generation of Multipotent Foregut Stem Cells from Human Pluripotent Stem Cells
    Hannan, Nicholas R. F.
    Fordham, Robert P.
    Syed, Yasir A.
    Moignard, Victoria
    Berry, Andrew
    Bautista, Ruben
    Hanley, Neil A.
    Jensen, Kim B.
    Vallier, Ludovic
    STEM CELL REPORTS, 2013, 1 (04): : 293 - 306
  • [49] Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells
    Hawkins, Finn J.
    Suzuki, Shingo
    Beermann, Mary Lou
    Barilla, Cristina
    Wang, Ruobing
    Villacorta-Martin, Carlos
    Berical, Andrew
    Jean, J. C.
    Le Suer, Jake
    Matte, Taylor
    Simone-Roach, Chantelle
    Tang, Yang
    Schlaeger, Thorsten M.
    Crane, Ana M.
    Matthias, Nadine
    Huang, Sarah X. L.
    Randell, Scott H.
    Wu, Joshua
    Spence, Jason R.
    Carraro, Gianni
    Stripp, Barry R.
    Rab, Andras
    Sorsher, Eric J.
    Horani, Amjad
    Brody, Steven L.
    Davis, Brian R.
    Kotton, Darrell N.
    CELL STEM CELL, 2021, 28 (01) : 79 - +
  • [50] Induced pluripotent stem (iPS) cells from human fetal stem cells
    Guillot, Pascale V.
    BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY, 2016, 31 : 112 - 120
12345