A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

被引：12
|
作者：
Dewi, Chitra Umala [1 ]
Mason, Michele [1 ]
Cohen-Hyams, Tzipi [2 ]
Killingsworth, Murray C. [2 ]
Harman, David G. [1 ]
Gnanasambandapillai, Vikkitharan [3 ]
Liyanage, Liwan [4 ]
O'Connor, Michael D. [1 ]
机构：
[1] Western Sydney Univ, Sch Med, Campbelltown, NSW 2560, Australia
[2] Ingham Inst, NSW Hlth Pathol & Correlat Microscopy Facil, Electron Microscopy Lab, Liverpool, NSW 2170, Australia
[3] Garvan Weizmann Ctr Cellular Genom, Darlinghurst, NSW 2010, Australia
[4] Western Sydney Univ, Sch Comp Data & Math Sci, Campbelltown, NSW 2560, Australia
来源：
EXPERIMENTAL EYE RESEARCH | 2021年 / 202卷
关键词：
Human; Lens; Lens epithelial cell; ROR1; Pluripotent stem cell; Micro-lens; Cataract; Single cell RNA-Sequencing; Bioinformatics;
D O I：
10.1016/j.exer.2020.108317
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of lightfocusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.
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收藏
页数：9
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