A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

被引：12

作者：

Dewi, Chitra Umala ^{[1
]}

Mason, Michele ^{[1
]}

Cohen-Hyams, Tzipi ^{[2
]}

Killingsworth, Murray C. ^{[2
]}

Harman, David G. ^{[1
]}

Gnanasambandapillai, Vikkitharan ^{[3
]}

Liyanage, Liwan ^{[4
]}

O'Connor, Michael D. ^{[1
]}

机构：

[1] Western Sydney Univ, Sch Med, Campbelltown, NSW 2560, Australia

[2] Ingham Inst, NSW Hlth Pathol & Correlat Microscopy Facil, Electron Microscopy Lab, Liverpool, NSW 2170, Australia

[3] Garvan Weizmann Ctr Cellular Genom, Darlinghurst, NSW 2010, Australia

[4] Western Sydney Univ, Sch Comp Data & Math Sci, Campbelltown, NSW 2560, Australia

来源：

EXPERIMENTAL EYE RESEARCH | 2021年 / 202卷

关键词：

Human; Lens; Lens epithelial cell; ROR1; Pluripotent stem cell; Micro-lens; Cataract; Single cell RNA-Sequencing; Bioinformatics;

D O I：

10.1016/j.exer.2020.108317

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of lightfocusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.

引用

页数：9

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