High-sensitivity mass spectrometry for analysis of posttranslational modifications

被引：5
|
作者：
Takao, T [1 ]
Shimizu, T [1 ]
Ikegami, S [1 ]
Shimonishi, Y [1 ]
机构：
[1] HIROSHIMA UNIV,DEPT APPL BIOCHEM,HIROSHIMA 739,JAPAN
来源：
JOURNAL OF PROTEIN CHEMISTRY | 1997年 / 16卷 / 05期
关键词：
pulsed-FAB; tandem mass spectrometry; high-energy collision-induced dissociation; histone modification; epsilon-(gamma-glutamyl)lysine;
D O I：
10.1023/A:1026388806194
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pulsed fast atom bombardment ionization (pulsed-FAB) mass spectrometry has been developed to improve the sensitivity of tandem mass spectrometry (MS/MS), allowing it to be used for the analysis of very small samples. MS/MS, when used with a magnetic four-sector instrument coupled with the pulsed-FAB system, allows significant enhancement in product ion intensity of over ten-fold in magnitude over conventional FAB. MS/MS was applied to the structural analysis of a unique nuclear protein, designated p28, which was isolated from a histone fraction obtained from starfish testes, The results clearly show that protein p28 is a heterodimer composed of testicular histones H2B and H4 which are cross-linked between Gln(9) of H2B and Lys(5) of H4.
引用
收藏
页码：409 / 413
页数：5
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