High-sensitivity mass spectrometry for analysis of posttranslational modifications

被引：5

作者：

Takao, T ^{[1
]}

Shimizu, T ^{[1
]}

Ikegami, S ^{[1
]}

Shimonishi, Y ^{[1
]}

机构：

[1] HIROSHIMA UNIV,DEPT APPL BIOCHEM,HIROSHIMA 739,JAPAN

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1997年 / 16卷 / 05期

关键词：

pulsed-FAB; tandem mass spectrometry; high-energy collision-induced dissociation; histone modification; epsilon-(gamma-glutamyl)lysine;

D O I：

10.1023/A:1026388806194

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pulsed fast atom bombardment ionization (pulsed-FAB) mass spectrometry has been developed to improve the sensitivity of tandem mass spectrometry (MS/MS), allowing it to be used for the analysis of very small samples. MS/MS, when used with a magnetic four-sector instrument coupled with the pulsed-FAB system, allows significant enhancement in product ion intensity of over ten-fold in magnitude over conventional FAB. MS/MS was applied to the structural analysis of a unique nuclear protein, designated p28, which was isolated from a histone fraction obtained from starfish testes, The results clearly show that protein p28 is a heterodimer composed of testicular histones H2B and H4 which are cross-linked between Gln(9) of H2B and Lys(5) of H4.

引用

页码：409 / 413

页数：5

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