Modeling retinitis pigmentosa through patient-derived retinal organoids

被引：13
|
作者：
Li, Yan-Ping [1 ,2 ]
Deng, Wen -Li [3 ,4 ]
Jin, Zi-Bing [1 ]
机构：
[1] Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr,Beijing Ophthalmol & Visua, Beijing 100730, Peoples R China
[2] Wenzhou Med Univ, Eye Hosp, Sch Ophthalmol & Optometry, Wenzhou 325027, Peoples R China
[3] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China
[4] Chongqing Eye Inst, Chongqing 400016, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
基金：
北京市自然科学基金;
关键词：
Cell Differentiation; Cell isolation; CRISPR; Organoids; Stem Cells;
D O I：
10.1016/j.xpro.2021.100438
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well -structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae.For complete details on the use and execution of this protocol, please refer to Deng et al. (2018).
引用
收藏
页数：20
相关论文
共 50 条
  • [41] Advances of Patient-Derived Organoids in Personalized Radiotherapy
    Wang, Yuenan
    Li, Ye
    Sheng, Zonghai
    Deng, Weiwei
    Yuan, Hongyan
    Wang, Shubin
    Liu, Yajie
    FRONTIERS IN ONCOLOGY, 2022, 12
  • [42] Patient-Derived Nasopharyngeal Cancer Organoids for Disease Modeling and Radiation Dose Optimization
    Lucky, Sasidharan Swarnalatha
    Law, Martin
    Lui, Ming Hong
    Mong, Jamie
    Shi, Junli
    Yu, Sidney
    Yoon, Do Kun
    Djeng, Shih Kien
    Wang, Jiguang
    Lim, Chwee Ming
    Tan, Min Han
    FRONTIERS IN ONCOLOGY, 2021, 11
  • [43] Modeling epithelial-mesenchymal transition in patient-derived breast cancer organoids
    Bar-Hai, Neta
    Ben-Yishay, Rakefet
    Arbili-Yarhi, Sheli
    Herman, Naama
    Avidan-Noy, Vered
    Menes, Tehillah
    Mansour, Aiham
    Awwad, Fahim
    Balint-Lahat, Nora
    Goldinger, Gil
    Hout-Siloni, Goni
    Adileh, Mohammad
    Berger, Raanan
    Ishay-Ronen, Dana
    FRONTIERS IN ONCOLOGY, 2024, 14
  • [44] Opportunities of patient-derived organoids in drug development
    Buening, Antonia
    Reckzeh, Elena
    BRITISH JOURNAL OF PHARMACOLOGY, 2025,
  • [45] Patient-derived organoids unveil sarcoma vulnerabilities
    Tiago Góss dos Santos
    Nature Reviews Cancer, 2024, 24 : 166 - 166
  • [46] Patient-derived organoids of malignant pleural mesothelioma
    Gonnelli, Francesca
    Volpini, Luca
    Monaco, Federica
    Tomasetti, Marco
    Barbisan, Francesca
    Goteri, Gaia
    Santarelli, Lory
    Ducatos, Lina
    Mei, Federico
    EUROPEAN RESPIRATORY JOURNAL, 2023, 62
  • [47] Patient-derived organoids in pediatric cancer research
    Calandrini, Camilla
    Schutgens, Frans
    Oka, Rurika
    Margaritis, Thanasis
    Candelli, Tito
    Mathijsen, Luka
    Ammerlaan, Carola
    van Ineveld, Ravian
    Derakhshan, Sepideh
    Custers, Lars
    Lijnzaad, Philip
    Begthel, Harry
    Kerstens, Hinri
    Rookmaker, Maarten
    Verhaar, Marianne
    Kemmeren, Patrick
    de Krijger, Ronald
    Pritchard-Jones, Kathy
    Rios, Anne
    van den Heuvel-Eibrink, Marry
    Holstege, Frank
    van Boxtel, Ruben
    Clevers, Hans
    Drost, Jarno
    CANCER RESEARCH, 2020, 80 (14) : 28 - 29
  • [48] Patient-derived organoids unveil sarcoma vulnerabilities
    dos Santos, Tiago Goss
    NATURE REVIEWS CANCER, 2024, 24 (03) : 166 - 166
  • [49] Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue
    Rodrigues, Amelie
    Slembrouck-Brec, Amelie
    Nanteau, Celine
    Terray, Angelique
    Tymoshenko, Yelyzaveta
    Zagar, Yvrick
    Reichman, Sacha
    Xi, Zhouhuan
    Sahel, Jose-Alain
    Fouquet, Stephane
    Orieux, Gael
    Nandrot, Emeline F.
    Byrne, Leah C.
    Audo, Isabelle
    Roger, Jerome E.
    Goureau, Olivier
    NPJ REGENERATIVE MEDICINE, 2022, 7 (01)
  • [50] Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue
    Amélie Rodrigues
    Amélie Slembrouck-Brec
    Céline Nanteau
    Angélique Terray
    Yelyzaveta Tymoshenko
    Yvrick Zagar
    Sacha Reichman
    Zhouhuan Xi
    José-Alain Sahel
    Stéphane Fouquet
    Gael Orieux
    Emeline F. Nandrot
    Leah C. Byrne
    Isabelle Audo
    Jérôme E. Roger
    Olivier Goureau
    npj Regenerative Medicine, 7
12345