Modeling retinitis pigmentosa through patient-derived retinal organoids

被引：13
|
作者：
Li, Yan-Ping [1 ,2 ]
Deng, Wen -Li [3 ,4 ]
Jin, Zi-Bing [1 ]
机构：
[1] Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr,Beijing Ophthalmol & Visua, Beijing 100730, Peoples R China
[2] Wenzhou Med Univ, Eye Hosp, Sch Ophthalmol & Optometry, Wenzhou 325027, Peoples R China
[3] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China
[4] Chongqing Eye Inst, Chongqing 400016, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
基金：
北京市自然科学基金;
关键词：
Cell Differentiation; Cell isolation; CRISPR; Organoids; Stem Cells;
D O I：
10.1016/j.xpro.2021.100438
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well -structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae.For complete details on the use and execution of this protocol, please refer to Deng et al. (2018).
引用
收藏
页数：20
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