Novel cap-independent translation with the 5′-noncoding region of L-A virus mRNA

被引：2
|
作者：
Ebihara, T [1 ]
Yanagida, Y [1 ]
Haruyama, T [1 ]
Kobatake, E [1 ]
Aizawa, M [1 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期
关键词：
L-A virus; mRNA; noncoding region; Saccharomyces cerevisiae; translation;
D O I：
10.1023/A:1005699202240
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel cap-independent translation has been performed where the ribosome entry is regulated by the 5'-noncoding region (NCR) of L-A virus mRNA. Despite L-A virus mRNA containing neither cap structure nor a poly(A) tail, the reconstructed mRNA encoding the 5' NCR of L-A virus mRNA and a reporter gene (luciferase) was translated, in yeast lysate, 60 times more efficiently than control mRNA. The 5' NCR from L-A virus was effective in regulating the recruitment of ribosome in vitro. A possible mechanism in Saccharomyces cerevisiae is also suggested, whereby the ribosome entry is regulated by the 5' NCR of L-A virus mRNA.
引用
收藏
页码：59 / 63
页数：5
相关论文
共 50 条
  • [41] New structural insights into cap-independent translation initiation
    Brilot, Axel
    Koh, Cha San
    Grigorieff, Nikolaus
    Korostelev, Andrei
    FASEB JOURNAL, 2014, 28 (01):
  • [42] Cap-independent translation of maize Hsp101
    Dinkova, TD
    Zepeda, H
    Martínez-Salas, E
    Martínez, LM
    Nieto-Sotelo, J
    de Jiménez, ES
    PLANT JOURNAL, 2005, 41 (05): : 722 - 731
  • [43] Cap-independent translation of heat shock messenger RNAs
    Rhoads, RE
    Lamphear, BJ
    CAP-INDEPENDENT TRANSLATION, 1995, 203 : 131 - 153
  • [44] Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region
    Andreev, D. E.
    Dmitriev, S. E.
    Terenin, I. M.
    Shatsky, I. N.
    BIOCHEMISTRY-MOSCOW, 2013, 78 (02) : 157 - 165
  • [45] Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region
    D. E. Andreev
    S. E. Dmitriev
    I. M. Terenin
    I. N. Shatsky
    Biochemistry (Moscow), 2013, 78 : 157 - 165
  • [46] DAP5 increases axonal outgrowth of hippocampal neurons by enhancing the cap-independent translation of DSCR1.4 mRNA
    Ji-Young Seo
    Youngseob Jung
    Do-Yeon Kim
    Hye Guk Ryu
    Juhyun Lee
    Sung Wook Kim
    Kyong-Tai Kim
    Cell Death & Disease, 10
  • [47] DAP5 increases axonal outgrowth of hippocampal neurons by enhancing the cap-independent translation of DSCR1.4 mRNA
    Seo, Ji-Young
    Jung, Youngseob
    Kim, Do-Yeon
    Ryu, Hye Guk
    Lee, Juhyun
    Kim, Sung Wook
    Kim, Kyong-Tai
    CELL DEATH & DISEASE, 2019, 10 (2)
  • [48] Mapping of sequences in the 5' region and 3' UTR of tomato ringspot virus RNA2 that facilitate cap-independent translation of reporter transcripts in vitro
    Paudel, Dinesh Babu
    Sanfacon, Helene
    PLOS ONE, 2021, 16 (04):
  • [49] LOW EFFICIENCY OF THE 5' NONTRANSLATED REGION OF HEPATITIS-A VIRUS-RNA IN DIRECTING CAP-INDEPENDENT TRANSLATION IN PERMISSIVE MONKEY KIDNEY-CELLS
    WHETTER, LE
    DAY, SP
    ELROYSTEIN, O
    BROWN, EA
    LEMON, SM
    JOURNAL OF VIROLOGY, 1994, 68 (08) : 5253 - 5263
  • [50] Analyzing the 5′-3′ Kissing-Loop Interaction Sequence for Cap-Independent Translation Initiation of Blackcurrant Reversion Virus
    Zhou, Liheng
    Lee, Julie
    Baquero-Galvis, Laura D.
    Filbin, Megan E.
    FASEB JOURNAL, 2022, 36
12345