Novel cap-independent translation with the 5′-noncoding region of L-A virus mRNA

被引：2

作者：

Ebihara, T ^{[1
]}

Yanagida, Y ^{[1
]}

Haruyama, T ^{[1
]}

Kobatake, E ^{[1
]}

Aizawa, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期

关键词：

L-A virus; mRNA; noncoding region; Saccharomyces cerevisiae; translation;

D O I：

10.1023/A:1005699202240

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel cap-independent translation has been performed where the ribosome entry is regulated by the 5'-noncoding region (NCR) of L-A virus mRNA. Despite L-A virus mRNA containing neither cap structure nor a poly(A) tail, the reconstructed mRNA encoding the 5' NCR of L-A virus mRNA and a reporter gene (luciferase) was translated, in yeast lysate, 60 times more efficiently than control mRNA. The 5' NCR from L-A virus was effective in regulating the recruitment of ribosome in vitro. A possible mechanism in Saccharomyces cerevisiae is also suggested, whereby the ribosome entry is regulated by the 5' NCR of L-A virus mRNA.

引用

页码：59 / 63

页数：5

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