Novel cap-independent translation with the 5′-noncoding region of L-A virus mRNA

被引：2
|
作者：
Ebihara, T [1 ]
Yanagida, Y [1 ]
Haruyama, T [1 ]
Kobatake, E [1 ]
Aizawa, M [1 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期
关键词：
L-A virus; mRNA; noncoding region; Saccharomyces cerevisiae; translation;
D O I：
10.1023/A:1005699202240
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel cap-independent translation has been performed where the ribosome entry is regulated by the 5'-noncoding region (NCR) of L-A virus mRNA. Despite L-A virus mRNA containing neither cap structure nor a poly(A) tail, the reconstructed mRNA encoding the 5' NCR of L-A virus mRNA and a reporter gene (luciferase) was translated, in yeast lysate, 60 times more efficiently than control mRNA. The 5' NCR from L-A virus was effective in regulating the recruitment of ribosome in vitro. A possible mechanism in Saccharomyces cerevisiae is also suggested, whereby the ribosome entry is regulated by the 5' NCR of L-A virus mRNA.
引用
收藏
页码：59 / 63
页数：5
相关论文
共 50 条
  • [1] Novel cap-independent translation with the 5′-noncoding region of L-A virus mRNA
    Takashi Ebihara
    Yasuko Yanagida
    Tetsuya Haruyama
    Eiry Kobatake
    Masuo Aizawa
    Biotechnology Letters, 2000, 22 : 59 - 63
  • [2] The 5'-untranslated region of the mouse mammary tumor virus mRNA exhibits cap-independent translation initiation
    Vallejos, Maricarmen
    Ramdohr, Pablo
    Valiente-Echeverria, Fernando
    Tapia, Karla
    Rodriguez, Felipe E.
    Lowy, Fernando
    Pablo Huidobro-Toro, J.
    Dangerfield, John A.
    Lopez-Lastra, Marcelo
    NUCLEIC ACIDS RESEARCH, 2010, 38 (02) : 618 - 632
  • [3] CAP-INDEPENDENT TRANSLATION BY THE 5' UNTRANSLATED REGION OF THEILERS MURINE ENCEPHALOMYELITIS VIRUS
    BANDYOPADHYAY, PK
    WANG, CY
    LIPTON, HL
    JOURNAL OF VIROLOGY, 1992, 66 (11) : 6249 - 6256
  • [4] Cap-independent translation conferred by the 5′-untranslated region of human thymidine kinase mRNA
    Chou, WL
    Chang, ZF
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2001, 1519 (03): : 209 - 215
  • [5] Cap-Independent Circular mRNA Translation Efficiency
    Deviatkin, Andrei A.
    Simonov, Ruslan A.
    Trutneva, Kseniya A.
    Maznina, Anna A.
    Soroka, Anastasiia B.
    Kogan, Anna A.
    Feoktistova, Sofya G.
    Khavina, Elena M.
    Mityaeva, Olga N.
    Volchkov, Pavel Y.
    VACCINES, 2023, 11 (02)
  • [6] Cap-Independent mRNA Translation in Germ Cells
    Keiper, Brett D.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (01):
  • [7] CAP-INDEPENDENT TRANSLATION OF POLIOVIRUS MESSENGER-RNA IS CONFERRED BY SEQUENCE ELEMENTS WITHIN THE 5' NONCODING REGION
    PELLETIER, J
    KAPLAN, G
    RACANIELLO, VR
    SONENBERG, N
    MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (03) : 1103 - 1112
  • [8] CAP-INDEPENDENT ENHANCEMENT OF TRANSLATION BY A PLANT POTYVIRUS 5' NONTRANSLATED REGION
    CARRINGTON, JC
    FREED, DD
    JOURNAL OF VIROLOGY, 1990, 64 (04) : 1590 - 1597
  • [9] The leader region of Laminin B1 mRNA confers cap-independent translation
    Petz, Michaela
    Kozina, Daniela
    Huber, Heidemarie
    Siwiec, Tanja
    Seipelt, Joachim
    Sommergruber, Wolfgang
    Mikulits, Wolfgang
    NUCLEIC ACIDS RESEARCH, 2007, 35 (08) : 2473 - 2482
  • [10] The 5′ Untranslated Region of the Human T-Cell Lymphotropic Virus Type 1 mRNA Enables Cap-Independent Translation Initiation
    Olivares, Eduardo
    Landry, Dori M.
    Joaquin Caceres, C.
    Pino, Karla
    Rossi, Federico
    Navarrete, Camilo
    Pablo Huidobro-Toro, Juan
    Thompson, Sunnie R.
    Lopez-Lastra, Marcelo
    JOURNAL OF VIROLOGY, 2014, 88 (11) : 5936 - 5955
12345