Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol

被引：16
|
作者：
Wilson, Eli Adjele [1 ]
Ennahar, Said [1 ]
Zhao, Minjie [1 ]
Bergaentzle, Martine [1 ]
Marchioni, Eric [1 ]
Bindler, Francoise [1 ]
机构：
[1] Univ Strasbourg, CNRS, Fac Pharm, Equipe Chim Analyt Mol Bioact,IPHC DSA, F-67400 Illkirch Graffenstaden, France
来源：
CHROMATOGRAPHIA | 2011年 / 73卷
关键词：
Column liquid chromatography; UV-detection; Mass spectrometry; Isothiocyanates; Solubility; Mercaptoethanol; PERFORMANCE LIQUID-CHROMATOGRAPHY; CRUCIFEROUS VEGETABLES; QUANTITATIVE-DETERMINATION; MASS-SPECTROMETRY; CABBAGE; CANCER; BROCCOLI; RAPESEED; PRODUCTS; INDOLES;
D O I：
10.1007/s10337-010-1878-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 mu g g(-1) fresh weight, respectively.
引用
收藏
页码：S137 / S142
页数：6
相关论文
共 50 条
  • [41] RP-LC Simultaneous Determination of Nebivolol Hydrochloride and Amlodipine Besilate in Bi-Layer Tablets
    Joshi, Sneha J.
    Karbhari, Pradnya A.
    Bhoir, Suvarna I.
    CHROMATOGRAPHIA, 2009, 70 (3-4) : 557 - 561
  • [42] Simultaneous Determination and Drug Dissolution Testing of Combined Amlodipine Tablet Formulations Using RP-LC
    Ozkan, Cansel Kose
    Kurbanoglu, Sevinc
    Esim, Ozgur
    Savaser, Ayhan
    Ozkan, Sibel A.
    Ozkan, Yalcin
    CHROMATOGRAPHIA, 2016, 79 (17-18) : 1143 - 1151
  • [43] Simultaneous Determination of Tranexamic Acid and Losartan Potassium in Dosage Formulations and Human Serum by RP-LC
    M. Saeed Arayne
    Najma Sultana
    Faiza Qureshi
    Farhan Ahmed Siddiqui
    Agha Zeeshan Mirza
    Saima Sher Bahadur
    Muhammad Hashim Zuberi
    Chromatographia, 2009, 70 : 789 - 795
  • [44] RP-LC Simultaneous Determination of Nebivolol Hydrochloride and Amlodipine Besilate in Bi-Layer Tablets
    Sneha J. Joshi
    Pradnya A. Karbhari
    Suvarna I. Bhoir
    Chromatographia, 2009, 70 : 557 - 561
  • [45] SPE for the simultaneous determination of various isothiocyanates
    Wilson, Eli Adjele
    Bindler, Francoise
    Marchioni, Eric
    Bergaentzle, Martine
    Benrabah, Tamime
    Ennahar, Said
    JOURNAL OF SEPARATION SCIENCE, 2012, 35 (23) : 3369 - 3374
  • [46] Stability Indicating RP-LC Method for the Determination of Gemifloxacin Mesylate
    Ranjane, Poonam N.
    Gandhi, Santosh V.
    Kadukar, Sayali S.
    Bothara, Kailash. G.
    CHROMATOGRAPHIA, 2010, 71 (11-12) : 1113 - 1117
  • [47] DETERMINATION OF ALLIIN IN GARLIC BY RP-HPLC ELECTROCHEMISTRY AND PRECOLUMN DERIVATIZATION
    ZIEGLER, SJ
    MEIER, B
    STICHER, O
    PHARMACEUTISCH WEEKBLAD-SCIENTIFIC EDITION, 1987, 9 (04) : 248 - 248
  • [48] Determination of Copper in Human Urine by RP-LC After Pre-column Derivatization with Neocuproine and Use of Monolithic Column
    Radoslav Halko
    Simona Procházková
    Lenka Okenicová
    Milan Hutta
    Peter Božek
    Chromatographia, 2014, 77 : 1019 - 1025
  • [49] Stability Indicating RP-LC Method for the Determination of Gemifloxacin Mesylate
    Poonam N. Ranjane
    Santosh V. Gandhi
    Sayali S. Kadukar
    Kailash. G. Bothara
    Chromatographia, 2010, 71 : 1113 - 1117
  • [50] Determination of Dimenhydrinate Nasal Delivery System in the Blood by RP-LC
    Cansel Köse Özkan
    Umut Taşdemir
    Çetin Taş
    Ayhan Savaşer
    Halil Erol
    Yalcın Özkan
    Chromatographia, 2013, 76 : 1521 - 1525
12345