Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol

被引：16
|
作者：
Wilson, Eli Adjele [1 ]
Ennahar, Said [1 ]
Zhao, Minjie [1 ]
Bergaentzle, Martine [1 ]
Marchioni, Eric [1 ]
Bindler, Francoise [1 ]
机构：
[1] Univ Strasbourg, CNRS, Fac Pharm, Equipe Chim Analyt Mol Bioact,IPHC DSA, F-67400 Illkirch Graffenstaden, France
来源：
CHROMATOGRAPHIA | 2011年 / 73卷
关键词：
Column liquid chromatography; UV-detection; Mass spectrometry; Isothiocyanates; Solubility; Mercaptoethanol; PERFORMANCE LIQUID-CHROMATOGRAPHY; CRUCIFEROUS VEGETABLES; QUANTITATIVE-DETERMINATION; MASS-SPECTROMETRY; CABBAGE; CANCER; BROCCOLI; RAPESEED; PRODUCTS; INDOLES;
D O I：
10.1007/s10337-010-1878-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 mu g g(-1) fresh weight, respectively.
引用
收藏
页码：S137 / S142
页数：6
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