Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol

被引：16
|
作者：
Wilson, Eli Adjele [1 ]
Ennahar, Said [1 ]
Zhao, Minjie [1 ]
Bergaentzle, Martine [1 ]
Marchioni, Eric [1 ]
Bindler, Francoise [1 ]
机构：
[1] Univ Strasbourg, CNRS, Fac Pharm, Equipe Chim Analyt Mol Bioact,IPHC DSA, F-67400 Illkirch Graffenstaden, France
来源：
CHROMATOGRAPHIA | 2011年 / 73卷
关键词：
Column liquid chromatography; UV-detection; Mass spectrometry; Isothiocyanates; Solubility; Mercaptoethanol; PERFORMANCE LIQUID-CHROMATOGRAPHY; CRUCIFEROUS VEGETABLES; QUANTITATIVE-DETERMINATION; MASS-SPECTROMETRY; CABBAGE; CANCER; BROCCOLI; RAPESEED; PRODUCTS; INDOLES;
D O I：
10.1007/s10337-010-1878-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 mu g g(-1) fresh weight, respectively.
引用
收藏
页码：S137 / S142
页数：6
相关论文
共 50 条
  • [1] Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
    Eli Adjélé Wilson
    Saïd Ennahar
    Minjie Zhao
    Martine Bergaentzle
    Eric Marchioni
    Françoise Bindler
    Chromatographia, 2011, 73 : 137 - 142
  • [2] Simultaneous RP-LC determination of additives in soft drinks
    Dossi, N.
    Toniolo, R.
    Susmel, S.
    Pizzariello, A.
    Bontempelli, G.
    CHROMATOGRAPHIA, 2006, 63 (11-12) : 557 - 562
  • [3] Simultaneous RP-LC Determination of Additives in Soft Drinks
    N. Dossi
    R. Toniolo
    S. Susmel
    A. Pizzariello
    G. Bontempelli
    Chromatographia, 2006, 63 : 557 - 562
  • [4] Simultaneous determination of zidovudine and nevirapine in human plasma by RP-LC
    Marchei, E
    Valvo, L
    Pacifici, R
    Pellegrini, M
    Tossini, G
    Zuccaro, P
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2002, 29 (06) : 1081 - 1088
  • [5] Simultaneous RP-LC Determination of Artesunate and Amodiaquine in Pharmaceutical Preparations
    Phadke, Manisha U.
    Jadhav, Vivek K.
    Jadhav, Rajendra K.
    Dave, Suraj S.
    Patil, Dattatray S.
    CHROMATOGRAPHIA, 2008, 68 (11-12) : 1003 - 1007
  • [6] Simultaneous RP-LC Determination of Artesunate and Amodiaquine in Pharmaceutical Preparations
    Manisha U. Phadke
    Vivek K. Jadhav
    Rajendra K. Jadhav
    Suraj S. Dave
    Dattatray S. Patil
    Chromatographia, 2008, 68 : 1003 - 1007
  • [7] Simultaneous Determination of Etodolac and Acetaminophen in Tablet Dosage Form by RP-LC
    Dongre, Vaijanath G.
    Shah, Sweta B.
    Bayes, Gunaji S.
    Phadke, Manisha
    Jadhav, Vivek K.
    CHROMATOGRAPHIA, 2009, 69 (9-10) : 1019 - 1023
  • [8] Simultaneous RP-LC Determination of Ketorolac and its Piperazinylalkyl Ester Prodrugs
    A. M. Qandil
    B. M. Tashtoush
    B. M. Al-Taani
    S. M. Al-Nabulsi
    F. Al-Zogoul
    Chromatographia, 2008, 67 : 287 - 291
  • [9] Simultaneous Determination of Nine Major Flavonoids in Sophora flavescens by RP-LC
    Xiao-Chi Ma
    Xiu-Lan Xin
    Ke-Xin Liu
    Bao-Jing Zhang
    Feng-Yun Li
    De-An Guo
    Chromatographia, 2008, 68 : 471 - 474
  • [10] Simultaneous RP-LC determination of ketorolac and its piperazinylalkyl ester prodrugs
    Qandil, A. M.
    Tashtoush, B. M.
    Al-Taani, B. M.
    Al-Nabulsi, S. M.
    Al-Zogoul, F.
    CHROMATOGRAPHIA, 2008, 67 (3-4) : 287 - 291
12345