Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

被引：6
|
作者：
Mori, Kento [1 ]
Sun, Hongkai [1 ]
Miura, Kazuki [1 ]
Simizu, Siro [1 ]
机构：
[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, 3-14-1 Hiyoshi, Yokohama, Kanagawa 2238522, Japan
来源：
MOLECULES | 2021年 / 26卷 / 18期
关键词：
C-mannosylation; glycobiology; myotube; myogenic differentiation; MUSCLE-CELL FUSION; C-MANNOSYLATION; ENZYMATIC-ACTIVITY; SECRETION; MANNOSYLTRANSFERASE; IDENTIFICATION; EXPRESSION; R-SPONDIN1; DPY-19;
D O I：
10.3390/molecules26185685
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.
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页数：9
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