Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

被引：6

作者：

Mori, Kento ^{[1
]}

Sun, Hongkai ^{[1
]}

Miura, Kazuki ^{[1
]}

Simizu, Siro ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, 3-14-1 Hiyoshi, Yokohama, Kanagawa 2238522, Japan

来源：

MOLECULES | 2021年 / 26卷 / 18期

关键词：

C-mannosylation; glycobiology; myotube; myogenic differentiation; MUSCLE-CELL FUSION; C-MANNOSYLATION; ENZYMATIC-ACTIVITY; SECRETION; MANNOSYLTRANSFERASE; IDENTIFICATION; EXPRESSION; R-SPONDIN1; DPY-19;

D O I：

10.3390/molecules26185685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

引用

页数：9

共 50 条

[21] Role of MiR-325-3p in the Regulation of CFL2 and Myogenic Differentiation of C2C12 Myoblasts
Mai Thi Nguyen
Lee, Wan
CELLS, 2021, 10 (10)
[22] Inhibition of Sesn2 has negative regulatory effects on the myogenic differentiation of C2C12 myoblasts
Song, Zubiao
Lin, Qing
Liang, Jiahui
Zhang, Weixi
MOLECULAR BIOMEDICINE, 2024, 5 (01):
[23] β-Taxilin participates in differentiation of C2C12 myoblasts into myotubes
Sakane, Hiroshi
Makiyama, Tomohiko
Nogami, Satoru
Horii, Yukimi
Akasaki, Kenji
Shirataki, Hiromichi
EXPERIMENTAL CELL RESEARCH, 2016, 345 (02) : 230 - 238
[24] EXPRESSION OF C-JUN/AP-1 DURING MYOGENIC DIFFERENTIATION IN MOUSE C2C12 MYOBLASTS
THINAKARAN, G
OJALA, J
BAG, J
FEBS LETTERS, 1993, 319 (03) : 271 - 276
[25] 3D-Structual Modeling of Myogenic Differentiation of C2C12 Myoblasts by Advanced Electron Microscopy and Light Microscopy
Ichinose, Takako M.
Saitoh, Sei
Iwane, Atsuko H.
BIOPHYSICAL JOURNAL, 2018, 114 (03) : 164A - 164A
[26] An siRNA-based screen in C2C12 myoblasts identifies novel genes involved in myogenic differentiation
Alwan, Rayan
Bruel, Ange-Line
Da Silva, Anne
Blanquet, Veronique
Bouhouche, Khaled
EXPERIMENTAL CELL RESEARCH, 2017, 359 (01) : 145 - 153
[27] Expression and Subcellular Localization of Myogenic Regulatory Factors During the Differentiation of Skeletal Muscle C2C12 Myoblasts
Ferri, Paola
Barbieri, Elena
Burattini, Sabrina
Guescini, Michele
D'Emilio, Alessandra
Biagiotti, Laura
Del Grande, Paolo
De Luca, Antonio
Stocchi, Vilberto
Falcieri, Elisabetta
JOURNAL OF CELLULAR BIOCHEMISTRY, 2009, 108 (06) : 1302 - 1317
[28] Twinfilin-1 is an essential regulator of myogenic differentiation through the modulation of YAP in C2C12 myoblasts
Mai Thi Nguyen
Won, You Han
Kwon, Tae Won
Lee, Wan
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022, 599 : 17 - 23
[29] Folic acid promotes the myogenic differentiation of C2C12 murine myoblasts through the Akt signaling pathway
Hwang, Seong Yeon
Kang, Yong Jung
Sung, Bokyung
Kim, Minjung
Kim, Dong Hwan
Lee, Yujin
Yoo, Mi-Ae
Kim, Cheol Min
Chung, Hae Young
Kim, Nam Deuk
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2015, 36 (04) : 1073 - 1080
[30] A role for PKCε during C2C12 myogenic differentiation
Gaboardi, Gian Carlo
Ramazzotti, Giulia
Bavelloni, Alberto
Piazzi, Manuela
Fiume, Roberta
Billi, Anna Maria
Matteucci, Alessandro
Faenza, Irene
Cocco, Lucio
CELLULAR SIGNALLING, 2010, 22 (04) : 629 - 635

← 1 2 3 4 5 →