A water-soluble two-photon ratiometric triarylboron probe with nucleolar targeting by preferential RNA binding

被引：49

作者：

Liu, Jun ^{[1
,2
]}

Zhang, Shilu ^{[1
,2
]}

Zhang, Chenghua ^{[1
,2
]}

Dong, Jun ^{[1
,2
]}

Shen, Chengyi ^{[1
,2
]}

Zhu, Jiang ^{[1
,2
]}

Xu, Huajun ^{[3
]}

Fu, Mingkai ^{[4
]}

Yang, Guoqiang ^{[5
]}

Zhang, Xiaoming ^{[6
]}

机构：

[1] North Sichuan Med Coll, Sch Preclin Med, Sichuan Key Lab Med Imaging, Nanchong 637000, Peoples R China

[2] North Sichuan Med Coll, Sch Preclin Med, Dept Chem, Nanchong 637000, Peoples R China

[3] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[4] Chinese Acad Sci, Inst Elect Engn, Beijing 100190, Peoples R China

[5] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Photochem, Beijing 100190, Peoples R China

[6] North Sichuan Med Coll, Affiliated Hosp, Nanchong 637000, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 83期

关键词：

FLUORESCENT-PROBES; LIVING CELLS; CYTOPLASM; COMPOUND; COMPLEX;

D O I：

10.1039/c7cc06045a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By functionalizing triarylboron with cyclen, we developed a two-photon fluorescence probe, TAB-2, which can selectively bind RNA with a ratiometric readout. We tested TAB-2 in NIH/3T3 fibroblast cells, and demonstrated its capability in visualizing nucleoli and analyzing microenvironment polarity by two-photon and fluorescence-lifetime imaging microscopy.

引用

页码：11476 / 11479

页数：4

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