Comparison of three methods of releasing DNA from Mycobacterium avium subsp Paratuberculosis cells

In the present study, three methods of releasing DNA from MAP cells were described. The first one consists in cell wall lysis with the use of sodium lauryl surface (SDS), NaOH and convertible application of low and high temperatures. In the second method, a mini bead-beater was used. In the third method, apart from using mini bead-beater DNA elution was performed additionally from the obtained dilution. The best results of DNA extraction from the MAP cells were obtained with the use of SDS and NaOH.