Comparison of three methods of releasing DNA from Mycobacterium avium subsp Paratuberculosis cells

被引：0
|
作者：
Fus, MM [1 ]
Szteyn, J
Wiszniewska, A
Herman, L
机构：
[1] Univ Warmia & Mazury, Fac Med Vet, Chair Anim Prod Hyg, PL-10957 Olsztyn, Poland
[2] Agr Res Ctr Ghent, Dept Anim Prod Qual, Melle, Belgium
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2003年 / 47卷 / 01期
关键词：
Mycobacterium avium subsp paratuberculosis; cell wall lysis; template DNA releasing; DNA elution;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the present study, three methods of releasing DNA from MAP cells were described. The first one consists in cell wall lysis with the use of sodium lauryl surface (SDS), NaOH and convertible application of low and high temperatures. In the second method, a mini bead-beater was used. In the third method, apart from using mini bead-beater DNA elution was performed additionally from the obtained dilution. The best results of DNA extraction from the MAP cells were obtained with the use of SDS and NaOH.
引用
收藏
页码：107 / 112
页数：6
相关论文
共 50 条
  • [31] Detection and Enumeration of Mycobacterium avium subsp paratuberculosis in Milk
    Herman, L
    Reybroeck, W
    D'Haese, E
    Zorman, T
    Nelis, H
    PROCEEDINGS OF THE SIXTH INTERNATIONAL COLLOQUIUM ON PARATUBERCULOSIS, 1999, : 543 - 552
  • [32] Identification of immunoreactive proteins of Mycobacterium avium subsp paratuberculosis
    Piras, Cristian
    Soggiu, Alessio
    Bonizzi, Luigi
    Greco, Viviana
    Ricchi, Matteo
    Arrigoni, Norma
    Bassols, Anna
    Urbani, Andrea
    Roncada, Paola
    PROTEOMICS, 2015, 15 (04) : 813 - 823
  • [33] Adsorption of Mycobacterium avium subsp paratuberculosis to Soil Particles
    Dhand, Navneet K.
    Toribio, Jenny-Ann L. M. L.
    Whittington, Richard J.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (17) : 5581 - 5585
  • [34] Genomic polymorphisms for Mycobacterium avium subsp paratuberculosis diagnostics
    Semret, M
    Alexander, DC
    Turenne, CY
    de Haas, P
    Overduin, P
    van Soolingen, D
    Cousins, D
    Behr, MA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) : 3704 - 3712
  • [35] ISOLATION OF MYCOBACTERIUM AVIUM subsp. PARATUBERCULOSIS FROM MOUFLON IN BULGARIA
    Savova, T.
    Petrova, R.
    Valcheva, V.
    Bonovska, M.
    Najdenski, H.
    INFEKTSIYA I IMMUNITET, 2019, 9 (5-6): : 665 - 670
  • [36] Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis
    Lefrancois L.H.
    Bodier C.C.
    Lecher S.
    Gilbert F.B.
    Cochard T.
    Harichaux G.
    Labas V.
    Teixeira-Gomes A.P.
    Raze D.
    Locht C.
    Biet F.
    BMC Research Notes, 6 (1)
  • [37] PCR variants of Mycobacterium avium subsp. paratuberculosis
    Nishimori, K
    Eguchi, M
    Tanaka, K
    Nakaoka, Y
    FIFTH INTERNATIONAL COLLOQUIUM ON PARATUBERCULOSIS: MEETING OF THE INTERNATIONAL ASSOCIATION FOR PARATUBERCULOSIS, 1997, : 114 - 114
  • [38] Mycobacterium avium subsp. paratuberculosis Virulence: A Review
    Ssekitoleko, Judah
    Ojok, Lonzy
    Abd El Wahed, Ahmed
    Erume, Joseph
    Amanzada, Ahmad
    Eltayeb, ElSagad
    Eltom, Kamal H.
    Okuni, Julius Boniface
    MICROORGANISMS, 2021, 9 (12)
  • [39] Heat inactivation of Mycobacterium avium subsp paratuberculosis in milk
    McDonald, WL
    O'Riley, KJ
    Schroen, CJ
    Condron, RJ
    PROCEEDINGS OF THE SEVENTH INTERNATIONAL COLLOQUIUM ON PARATUBERCULOSIS, 2003, : 312 - 316
  • [40] Mycobacterium avium subsp paratuberculosis and Crohn's disease
    Naser, Saleh A.
    Naser, Najih A.
    EMERGING INFECTIONS 7, 2007, 7 : 225 - +
12345