Comparison of three methods of releasing DNA from Mycobacterium avium subsp Paratuberculosis cells

被引：0
|
作者：
Fus, MM [1 ]
Szteyn, J
Wiszniewska, A
Herman, L
机构：
[1] Univ Warmia & Mazury, Fac Med Vet, Chair Anim Prod Hyg, PL-10957 Olsztyn, Poland
[2] Agr Res Ctr Ghent, Dept Anim Prod Qual, Melle, Belgium
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2003年 / 47卷 / 01期
关键词：
Mycobacterium avium subsp paratuberculosis; cell wall lysis; template DNA releasing; DNA elution;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the present study, three methods of releasing DNA from MAP cells were described. The first one consists in cell wall lysis with the use of sodium lauryl surface (SDS), NaOH and convertible application of low and high temperatures. In the second method, a mini bead-beater was used. In the third method, apart from using mini bead-beater DNA elution was performed additionally from the obtained dilution. The best results of DNA extraction from the MAP cells were obtained with the use of SDS and NaOH.
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收藏
页码：107 / 112
页数：6
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