Localization of Apafl1 gene expression in the early development of the mouse by means of in situ reverse transcriptase-polymerase chain reaction

被引:9
|
作者
Müller, M
Berger, J
Gersdorff, N
Cecconi, F
Herken, R
Quondamatteo, F
机构
[1] Univ Gottingen, Dept Prosthodont, D-37075 Gottingen, Germany
[2] Univ Gottingen, Dept Histol, D-37075 Gottingen, Germany
[3] MPI Biophys Chem, Dept Mol Biol, Gottingen, Germany
[4] Univ Roma Tor Vergata, Dept Biol, Dulbecco Telethon Inst, Rome, Italy
[5] IRCCS, Fdn Santa Lucia, CERC, Rome, Italy
关键词
apoptosis; early development; Apaf1; in situ RT-PCR;
D O I
10.1002/dvdy.20534
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Apoptosis is an essential ubiquitous process that controls the duration of the life span of cells, thus playing a crucial role in morphogenetic, histogenetic, and phylogenetic developmental processes. Apaf1 (apoptosis protease activating factor 1) is one of the central mediators of the intrinsic apoptotic pathway and a part of the apoptosome, which activates procaspase-3 and promotes cell death. Gene knockout of Apaf1 in mice leads to late embryonic lethality with malformations such as the persistence of interdigital webs and hyperplasia of brain and retina. Therefore, Apaf1 is generally believed to play a crucial role in developmental apoptosis and have a widespread expression. However, its pattern of expression in early development remains unknown. To specify whether Apaf1 indeed plays this key role, we investigated the pattern of gene expression for Apaf1 in mouse embryos on day 7,9, and 12 of development. Our results show, that gene expression for Apafl first occurs within the embryo between day 7 and 9 of development, becoming more widespread toward day 12 and then includes structures, such as yolk sac, mesenchyme, cartilage, heart anlage, otic vesicle, peridermis, and anlagen of the spinal ganglia and vertebral bodies. Our results also show that gene expression for Apaf1 is not ubiquitous in early mouse development. This finding indicates that cell death processes are independent of or less dependent on Apaf1 during this time. Of interest, an active gene expression for Apafl is also present in organ anlagen such as heart or intestine, in which no obvious phenotype is seen after Apafl deletion. This finding suggests a possible role for Apafl in such anlagen as a putative alternative compensatory pathway, which could he switched on in the case of defects in the mediators that are normally involved in such organs. (c) 2005 Wiley-Liss, Inc.
引用
收藏
页码:215 / 221
页数:7
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