Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction

被引：23
|
作者：
Miagostovich, MP [1 ]
dosSantos, FB [1 ]
deAraujo, ESM [1 ]
Dias, J [1 ]
Schatzmayr, HG [1 ]
Nogueira, RMR [1 ]
机构：
[1] SECRETARIA SAUDE ESTADO BAHIA, DIV VIGILANCIA EPIDEMIOL, SALVADOR, BA, BRAZIL
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1997年 / 92卷 / 05期
关键词：
dengue; diagnosis; virological surveillance; polymerase chain reaction;
D O I：
10.1590/S0074-02761997000500006
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.
引用
收藏
页码：595 / 599
页数：5
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