Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction

被引：23

作者：

Miagostovich, MP ^{[1
]}

dosSantos, FB ^{[1
]}

deAraujo, ESM ^{[1
]}

Dias, J ^{[1
]}

Schatzmayr, HG ^{[1
]}

Nogueira, RMR ^{[1
]}

机构：

[1] SECRETARIA SAUDE ESTADO BAHIA, DIV VIGILANCIA EPIDEMIOL, SALVADOR, BA, BRAZIL

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1997年 / 92卷 / 05期

关键词：

dengue; diagnosis; virological surveillance; polymerase chain reaction;

D O I：

10.1590/S0074-02761997000500006

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.

引用

页码：595 / 599

页数：5

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