Rapid Determination of Vitamins D2 and D3 in Dairy Products by High-Performance Liquid Chromatography after Solid Phase Extraction Based on Polystyrene/Polypyrrole Composite Nanofibers

被引:3
|
作者
Han, Qing [1 ]
Wei, Lanlan [2 ]
Deng, Guozhe [1 ]
Sun, Ying [1 ]
Deng, Jianjun [3 ]
Tang, Zigang [2 ]
Fan, Jinhui [1 ]
Wang, Xia [1 ]
Kang, Xuejun [1 ]
机构
[1] Southeast Univ, Sch Biol Sci & Med Engn, Minist Educ, Res Ctr Learning Sci,Key Lab Child Dev & Learning, Nanjing 210096, Peoples R China
[2] Southeast Univ, Sch Publ Hlth, Minist Educ, Key Lab Environm Med & Engn, Nanjing 210096, Peoples R China
[3] Suzhou Dongqi Biol Technol Co, Suzhou 215123, Peoples R China
基金
中国国家自然科学基金;
关键词
nanofibers; solid-phase extraction; vitamin D-2; vitamin D-3; dairy products; FAT-SOLUBLE VITAMINS; MILK; SUPPLEMENTS; PARTICLES; DISEASES; REMOVAL; FIBER;
D O I
10.21577/0103-5053.20200007
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem. packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D-2 (D-2) and vitamin D-3 (D-3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL(-1) in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D-2 and D-3 in dairy products.
引用
收藏
页码:1216 / 1224
页数:9
相关论文
共 50 条
  • [41] Rapid determination of methotrexate in plasma by high-performance liquid chromatography with on-line solid-phase extraction and automated precolumn derivatization
    Emara, S
    Askal, H
    Masujima, T
    BIOMEDICAL CHROMATOGRAPHY, 1998, 12 (06) : 338 - 342
  • [42] Rapid determination of methotrexate in plasma by high-performance liquid chromatography with on-line solid-phase extraction and automated precolumn derivatization
    Emara, Samy
    Askal, Hassan
    Masujima, Tsutomu
    Biomedical Chromatography, 12 (06): : 338 - 342
  • [43] RAPID-DETERMINATION OF AFLATOXIN-M1 IN DAIRY-PRODUCTS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    FREMY, JM
    BOURSIER, B
    JOURNAL OF CHROMATOGRAPHY, 1981, 219 (01): : 156 - 161
  • [44] Determination of vitamin D3 in pharmaceutical products using salting-out assisted liquid-liquid extraction coupled with reversed phase liquid chromatography
    Abu Sa'aleek, Asmaa
    Alshishani, Anas
    Shaghlil, Lena
    Alhesan, Jameel S. Aljariri
    Al-ebini, Yousef
    MICROCHEMICAL JOURNAL, 2023, 193
  • [45] Determination of vitamin D in calcium fortified foods using solid-phase extraction-high performance liquid chromatography
    Zhao Rong
    Xue Ying
    Wu Guohua
    Zhao Haiyan
    Luo Rencai
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2008, 26 (01) : 113 - 115
  • [46] A Simple, Rapid Atmospheric Pressure Chemical Ionization Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of 25-Hydroxyvitamin D2 and D3
    Garg, Uttam
    Munar, Ada
    Frazee, Clinton, III
    Scott, David
    JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2012, 26 (05) : 349 - 357
  • [47] Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmacokinetic study
    Wang, HL
    Zou, HF
    Kong, L
    Zhang, YK
    Pang, H
    Su, CY
    Liu, GY
    Hui, M
    Fu, L
    JOURNAL OF CHROMATOGRAPHY B, 1999, 731 (02): : 403 - 409
  • [48] Determination of glycolic acid in cosmetic products by solid-phase extraction and reversed-phase ion-pair high-performance liquid chromatography
    Scalia, S
    Callegari, R
    Villani, S
    JOURNAL OF CHROMATOGRAPHY A, 1998, 795 (02) : 219 - 225
  • [49] Exogenous versus endogenous recovery of 25-hydroxyvitamins D2 and D3 in human samples using high-performance liquid chromatography and the DiaSorin LIAISON Total-D Assay
    Horst, Ronald L.
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2010, 121 (1-2): : 180 - 182
  • [50] Determination of Environmental Estrogens by Polybenzothiophene-based Solid-phase Extraction (SPE) and High-Performance Liquid Chromatography (HPLC)
    Rao, Danmei
    Yang, Chuan
    Liu, Shunxin
    Wang, Han
    Hu, Xiaorong
    ANALYTICAL LETTERS, 2024, 57 (01) : 41 - 57