Oral immunization against enterotoxigenic colibacillosis in weaned piglets by non-pathogenic Escherichia coli strain with K88 (F4) colonizing factors

被引:2
|
作者
Alexa, P [1 ]
Hamrik, J [1 ]
Stouracova, K [1 ]
Salajka, E [1 ]
机构
[1] Vet Res Inst, Hudcova 70, CS-62132 Brno, Czech Republic
关键词
piglet; diarrhoea after weaning; mucosal immunity; F4; fimbriae; K88;
D O I
10.17221/5629-VETMED
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Experiments were focused on the prevention of diarrhoea in weaned piglets by means of enterotoxigenic strains of Escherichia coli ( ETEC) with colonizing factors K88 (F4). The process of immunization consisted of intramuscular administration of ETEC strain bacterin one day prior to weaning and oral administration of a live culture of non-pathogenic E. coli strain containing colonizing factors (O149:K88; STa-, LT-) in 3 hours after weaning. The shedding of the K88 positive E. coli strains was monitored for 3 weeks after weaning by the culture of rectal swabs. The efficacy of such immunization protocol was tested by challenge exposure to enterotoxigenic E. coli O149: K88, LT+ strain on the third or the tenth day after weaning. Following the oral administration of non-pathogenic E. coli strain containing colonizing factors K88 to piglets, the shedding of the administered strain continued for 9 days. No or very small protection against diarrhoea following the challenge exposure to enterotoxigenic E. coli was found in immunized piglets.
引用
收藏
页码:315 / 320
页数:6
相关论文
共 50 条
  • [21] Dietary supplementation with Clostridium butyricum helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic Escherichia coli K88
    Li, H. -H.
    Li, Y. -P.
    Zhu, Q.
    Qiao, J. -Y.
    Wang, W. -J.
    JOURNAL OF APPLIED MICROBIOLOGY, 2018, 125 (04) : 964 - 975
  • [22] Real-time PCR for differentiation of F4 (K88) variants (F4ab, F4ac, F4ad) of enterotoxigenic Escherichia coli from diarrhoeic piglets
    Byun, Jae-Won
    Jung, Byeong Yea
    Kim, Ha-Young
    Fairbrother, John M.
    Lee, Wan-Kyu
    VETERINARY JOURNAL, 2012, 193 (02): : 593 - 594
  • [23] Biological response of piglets challenged with Escherichia coli F4 (K88) when fed diets containing intestinal alkaline phosphatase
    Genova, Jansller Luiz
    Rupolo, Paulo Evaristo
    Brandao Melo, Antonio Diego
    de Azevedo dos Santos, Liliana Bury
    Wendt, Geraldyne Nunes
    Barbosa, Keila Abadia
    Carvalho, Silvana Teixeira
    Escocard de Oliveira, Newton Tavares
    Costa, Leandro Batista
    de Oliveira Carvalho, Paulo Levi
    CZECH JOURNAL OF ANIMAL SCIENCE, 2021, 66 (10) : 391 - 402
  • [24] Enteric-coated pellets of F4 fimbriae for oral vaccination of suckling piglets against enterotoxigenic Escherichia coli infections
    Snoeck, V
    Huyghebaert, N
    Cox, E
    Vermeire, A
    Vancaeneghem, S
    Remon, JP
    Goddeeris, BM
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2003, 96 (3-4) : 219 - 227
  • [25] Probiotic supplementation protects weaned pigs against enterotoxigenic Escherichia coli K88 challenge and improves performance similar to antibiotics
    Pan, L.
    Zhao, P. F.
    Ma, X. K.
    Shang, Q. H.
    Xu, Y. T.
    Long, S. F.
    Wu, Y.
    Yuan, F. M.
    Piao, X. S.
    JOURNAL OF ANIMAL SCIENCE, 2017, 95 (06) : 2627 - 2639
  • [26] Dietary tryptophan improves growth and intestinal health by promoting the secretion of intestinal β-defensins against enterotoxigenic Escherichia coli F4 in weaned piglets
    Chen, Chen
    Hu, Hong
    Li, Zhangcheng
    Qi, Min
    Qiu, Yibin
    Hu, Zhijin
    Feng, Fu
    Tang, Wenjie
    Diao, Hui
    Sun, Weizhong
    Tang, Zhiru
    JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2024, 129
  • [27] Modulatory Effects of Vasoactive Intestinal Peptide on Intestinal Mucosal Immunity and Microbial Community of Weaned Piglets Challenged by an Enterotoxigenic Escherichia coli (K88)
    Xu, Chunlan
    Wang, Youming
    Sun, Rui
    Qiao, Xiangjin
    Shang, Xiaoya
    Niu, Weining
    PLOS ONE, 2014, 9 (08):
  • [28] Development and evaluation of an ELISA to detect Escherichia coli K88 (F4) fimbrial antibody levels
    Vazquez, F
    Gonzalez, EA
    Garabal, JI
    Valderrama, S
    Blanco, J
    Baloda, SB
    JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (06) : 453 - 463
  • [29] Protection of piglets against enteric colibacillosis by intranasal immunization with K88ac (F4ac) fimbriae and heat labile enterotoxin of Escherichia coli
    Lin, Jun
    Mateo, Kristina S.
    Zhao, Mojun
    Erickson, Alan K.
    Garcia, Nuria
    He, Dong
    Moxley, Rodney A.
    Francis, David H.
    VETERINARY MICROBIOLOGY, 2013, 162 (2-4) : 731 - 739
  • [30] Immunogenicity and protective efficacy of a single-dose live non-pathogenic Escherichia coli oral vaccine against F4-positive enterotoxigenic Escherichia coli challenge in pigs
    Fairbrother, John Morris
    Nadeau, Eric
    Belanger, Louise
    Tremblay, Cindy-Love
    Tremblay, Danielle
    Brunelle, Melanie
    Wolf, Regina
    Hellmann, Klaus
    Hidalgo, Alvaro
    VACCINE, 2017, 35 (02) : 353 - 360