Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme

被引：64
|
作者：
Tian, Tian [1 ]
Xiao, Heng [1 ]
Long, Yuelin [1 ]
Zhang, Xiaoe [1 ]
Wang, Shaoru [1 ]
Zhou, Xiang [1 ,2 ]
Liu, Songmei [3 ]
Zhou, Xin [3 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Hubei, Peoples R China
[2] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
[3] Wuhan Univ, Zhongnan Hosp, Wuhan 430072, Hubei, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 80期
关键词：
LABEL-FREE; DAM METHYLTRANSFERASE; COLORIMETRIC ASSAY; ESCHERICHIA-COLI; METHYLATION; SEQUENCE; IONS; IMMUNODETECTION; MOLECULES; BEACONS;
D O I：
10.1039/c2cc35648a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive analysis of MTase activity based on a hairpin shaped DNAzyme; 8-17 DNAzyme amplicon has been adopted and found to be very effective in such analysis.
引用
收藏
页码：10031 / 10033
页数：3
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