A single-molecule sensitive DNA hairpin system based on intramolecular electron transfer

被引：81
|
作者：
Piestert, O
Barsch, H
Buschmann, V
Heinlein, T
Knemeyer, JP
Weston, KD
Sauer, M
机构：
[1] Univ Heidelberg, Inst Phys Chem, D-69120 Heidelberg, Germany
[2] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA
来源：
NANO LETTERS | 2003年 / 3卷 / 07期
关键词：
D O I：
10.1021/nl0341988
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We describe a method for detection of sub-picomolar concentrations of DNA or RNA sequences using novel surface-immobilized DNA hairpins. Within the DNA hairpins a fluorophore is specifically quenched by guanosine residues in the complementary stem sequence via photoinduced intramolecular electron transfer. Upon hybridization to the target sequence, fluorescence is restored due to a conformational reorganization that forces the stem apart. Proper immobilization of the DNA hairpins using blotin/streptavidin binding with minimal perturbation of the surface is required to ensure efficient quenching in the closed state.
引用
收藏
页码：979 / 982
页数：4
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