Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches

被引:3
|
作者
Fujimori, H
Miura, S
Koseki, S
Hokari, R
Tsuzuki, Y
Komoto, S
Hara, Y
Suzuki, H
Serizawa, H
Ishii, H
机构
[1] Natl Def Med Coll, Dept Internal Med 2, Tokorozawa, Saitama 3598513, Japan
[2] Keio Univ, Sch Med, Dept Internal Med, Tokyo, Japan
[3] Kitasato Inst Hosp, Dept Internal Med, Tokyo, Japan
关键词
CINC/gro; T lymphocytes; Peyer's patch; postcapillary venules; transendothelial migration; collecting lymphatics;
D O I
10.1159/000051919
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: Cytokine-induced neutrophil chemoattractant (CINC/gro), a member of interleukin-8 family, was found as a potent chemotactic factor for rat neutrophils. Although several chemokines have been shown to be potent regulators of T cell chemotaxis in vitro, the potential role of chemokines in T-cell migration in gut-associated lymphoid tissues has not been investigated in vivo. in the present study, the effects of CINC/gro on T-lymphocyte migration were examined in rat Peyer's patches. Methods: T lymphocytes collected from intestinal lymph of rats were fluorescence-labeled and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy and the effects of CINC/gro infusion was investigated. Lymphocyte flux in mesenteric collecting lymphatics was also observed. Results: In vivo infusion of CINC/gro significantly attenuated the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, once these lymphocytes adhered to venules, CINC/gro treatment significantly accelerated the transendothelial migration of T lymphocytes and they also significantly increased their subsequent flux in collecting lymphatics. Conclusion: There is a possibility that CINC/gro could modulate the characteristics of T lymphocyte homing in the inflammatory sites of gut. Copyright (C) 2001 S. Karger AG, Basel.
引用
收藏
页码:97 / 102
页数:6
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