Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter

被引：38
|
作者：
Kostov, Y
Harms, P
Pilato, RS
Rao, G
机构：
[1] Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Baltimore, MD 21201 USA
[2] LUMET, Silver Spring, MD 20905 USA
来源：
ANALYST | 2000年 / 125卷 / 06期
关键词：
D O I：
10.1039/b002348p
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new method and device for the ratiometric measurement of oxygen concentration are presented. They are based on the use of a dual-emission oxygen-sensitive dye. The method allows the exclusion of the influence of emission overlap. The detection of the dual-emission ratio is performed using a single long-pass emission filter. The device described is simpler than the widely used lifetime instruments and could easily be a stand-alone low-cost instrument.
引用
收藏
页码：1175 / 1178
页数：4
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